Measurement of the Activity of the Atg4 Cysteine Proteases.
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ABSTRACT:
SUBMITTER: Li M
PROVIDER: S-EPMC5582973 | biostudies-literature | 2017
REPOSITORIES: biostudies-literature
Publications
Measurement of the Activity of the Atg4 Cysteine Proteases.
Li M M Fu Y Y Yang Z Z Yin X-M XM
Methods in enzymology 20161205
While only one Atg4 is present in yeast, there are four Atg4 homologues in human and in mouse with different substrate specificities and catalytic efficiencies. The molecule Atg4 is a type of cysteine protease, and is known for its crucial roles in cleavage of the Atg8 family proteins before they can be conjugated to phospholipids, and also in cleavage of the conjugated Atg8 molecules from the membrane, a process known as deconjugation. Both processes are required for the maximal efficiency in a ...[more]
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