Ontology highlight
ABSTRACT:
SUBMITTER: Amlinger L
PROVIDER: S-EPMC5583386 | biostudies-literature | 2017 Sep
REPOSITORIES: biostudies-literature
Scientific reports 20170904 1
CRISPR-Cas systems are adaptive prokaryotic immune systems protecting against horizontally transferred DNA or RNA such as viruses and other mobile genetic elements. Memory of past invaders is stored as spacers in CRISPR loci in a process called adaptation. Here we developed a novel assay where spacer integration results in fluorescence, enabling detection of memory formation in single cells and quantification of as few as 0.05% cells with expanded CRISPR arrays in a bacterial population. Using t ...[more]