Dataset Information

MRNA in exosomas as a liquid biopsy in non-Hodgkin Lymphoma: a multicentric study by the Spanish Lymphoma Oncology Group.

ABSTRACT:

Purpose

To determine the feasibility of mRNAs (C-MYC, BCL-XL, BCL-6, NF-??, PTEN and AKT) in exosomes of plasma as a liquid biopsy method for monitoring and prognostic evolution in B-cell lymphomas.

Patients and methods

Exosomes were isolated from 98 patients with B-cell Lymphoma and 68 healthy controls. mRNAs were analyzed by quantitative PCR. An additional 31 post-treatment samples were also studied.

Results

In the general and follicular lymphoma series, the presence of AKT mRNA was associated with poor response to rituximab-based treatment. Patients with first relapse or disease progression showed a lower percentage of PTEN and BCL-XL mRNA. The presence of BCL-6 mRNA was associated with a high death rate. The absence of PTEN mRNA in the general series, and presence of C-MYC mRNA in follicular lymphomas, were associated with short progression-free survival. BCL-6 and C-MYC mRNA were independent prognostic variables of overall survival. C-MYC mRNA may provide prognostic information with respect to overall survival. BCL-XL mRNA and increase of BCL-6 mRNA in post-treatment samples could serve as molecular monitoring markers.

Conclusions

This is the first large study to evaluate the prognostic and predictive values of pretreatment tumor-associated mRNA in exosomes. BCL-6 and C-MYC mRNA positivity in pretreatment samples were predictors of worse PFS compared to patients with mRNA negativity. C-MYC mRNA positivity was also a statistically significant predictor of inability to obtain complete response with first-line therapy.

SUBMITTER: Provencio M

PROVIDER: S-EPMC5584220 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature

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Publications

mRNA in exosomas as a liquid biopsy in non-Hodgkin Lymphoma: a multicentric study by the Spanish Lymphoma Oncology Group.

Provencio Mariano M Rodríguez Marta M Cantos Blanca B Sabín Pilar P Quero Cristina C García-Arroyo Francisco R FR Rueda Antonio A Maximiano Constanza C Rodríguez-Abreu Delvys D Sánchez Antonio A Silva Javier J García Vanesa V

Oncotarget 20170322 31

<h4>Purpose</h4>To determine the feasibility of mRNAs (<i>C-MYC, BCL-XL, BCL-6, NF-κβ, PTEN</i> and <i>AKT</i>) in exosomes of plasma as a liquid biopsy method for monitoring and prognostic evolution in B-cell lymphomas.<h4>Patients and methods</h4>Exosomes were isolated from 98 patients with B-cell Lymphoma and 68 healthy controls. mRNAs were analyzed by quantitative PCR. An additional 31 post-treatment samples were also studied.<h4>Results</h4>In the general and follicular lymphoma series, the ...[more]

PMID: 28881619

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