Dataset Information

Identification and characterization of microsatellite loci in the tuliptree, Liriodendron tulipifera (Magnoliaceae).

ABSTRACT: PREMISE OF THE STUDY:Twenty-three polymorphic microsatellite loci (simple sequence repeats) were identified and characterized for Liriodendron tulipifera (Magnoliaceae), a species native to eastern North America, to investigate its genetic diversity, population structure, and mating system. METHODS AND RESULTS:Using Illumina HiSeq paired-end reads from genomic DNA, searches for repeat motifs identified approximately 280,000 potentially amplifiable loci. Of 77 loci tested, 51 amplified consistently. When genotyped using 30 to 52 total adult trees from three old-growth populations in Maryland, Virginia, and New Jersey, USA, 23 loci were polymorphic. These loci exhibited four to 13 alleles, and observed and expected heterozygosities ranged from 0.233 to 0.865 and 0.272 to 0.876, respectively. CONCLUSIONS:The microsatellite marker loci presented here will be valuable in population genetic studies of L. tulipifera because they do not suffer from ascertainment bias and show high polymorphism.

SUBMITTER: Gutierrez-Ozuna R

PROVIDER: S-EPMC5584817 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature

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Identification and characterization of microsatellite loci in the tuliptree, <i>Liriodendron tulipifera</i> (Magnoliaceae).

Gutiérrez-Ozuna Ricardo R Hamilton Matthew B MB

Applications in plant sciences 20170824 8

<h4>Premise of the study</h4>Twenty-three polymorphic microsatellite loci (simple sequence repeats) were identified and characterized for <i>Liriodendron tulipifera</i> (Magnoliaceae), a species native to eastern North America, to investigate its genetic diversity, population structure, and mating system.<h4>Methods and results</h4>Using Illumina HiSeq paired-end reads from genomic DNA, searches for repeat motifs identified approximately 280,000 potentially amplifiable loci. Of 77 loci tested, 5 ...[more]

PMID: 28924513

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Project description:Flower colour and colour patterns are crucial traits for ornamental species; thus, a comprehensive understanding of their genetic basis is extremely significant for plant breeders. The tulip tree (Liriodendron tulipifera Linn.) is well known for its flowers, odd leave shape and tree form. However, the genetic basis of its colour inheritance remains unknown. In this study, a putative plastid terminal oxidase gene (LtuPTOX) was identified from L. tulipifera based on multiple databases of differentially expressed genes at various developmental stages. Then, the full-length cDNA of LtuPTOX was derived from tepals and leaves using RACE (rapid amplification of cDNA ends) approaches. Furthermore, gene structure and phylogenetic analyses of PTOX as well as AOXs (alternative oxidases), another highly similar homologue in the AOX family, were used to distinguish between the two subfamilies of genes. In addition, transient transformation and qPCR methods were used to determine the subcellular localization and tissue expression pattern of the LtuPTOX gene. Moreover, the expression of LtuPTOX as well as pigment contents was investigated to illustrate the function of this gene during the formation of orange bands on petals. The results showed that the LtuPTOX gene encodes a 358-aa protein that contains a complete AOX domain (PF01786). Accordingly, the Liriodendron PTOX and AOX genes were identified as only paralogs since they were rather similar in sequence. LtuPTOX showed chloroplast localization and was expressed in coloured organs such as petals and leaves. Additionally, an increasing pattern of LtuPTOX transcripts leads to carotenoid accumulation on the orange-band during flower bud development. Taken together, our results suggest that LtuPTOX is involved in petal carotenoid metabolism and orange band formation in L. tulipifera. The identification of this potentially involved gene will lay a foundation for further uncovering the genetic basis of flower colour in L. tulipifera.

Dataset Information

Identification and characterization of microsatellite loci in the tuliptree, Liriodendron tulipifera (Magnoliaceae).

Publications

Identification and characterization of microsatellite loci in the tuliptree, <i>Liriodendron tulipifera</i> (Magnoliaceae).

Similar Datasets

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