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Isolation and characterization of microsatellite loci from Arthropodium cirratum (Asparagaceae).

ABSTRACT: PREMISE OF THE STUDY:Microsatellite markers were developed for Arthropodium cirratum (Asparagaceae) to study population genetic structure and translocation of this species. These markers were tested for cross-amplification in two other Arthropodium species. METHODS AND RESULTS:Sixteen microsatellite markers were developed from a genomic library and tested in three populations of A. cirratum. The loci exhibited one to five alleles per locus, with private alleles present in each of the populations. Cross-amplification tests in the two other New Zealand Arthropodium species revealed that many of the loci amplify and demonstrate polymorphism in A. bifurcatum. CONCLUSIONS:These markers will be useful for determining genetic structure in A. cirratum and for determining the origins of translocated populations of this species.

SUBMITTER: Bulgarella M

PROVIDER: S-EPMC5584818 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature

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Isolation and characterization of microsatellite loci from <i>Arthropodium cirratum</i> (Asparagaceae).

Bulgarella Mariana M Biggs Patrick J PJ de Lange Peter J PJ Shepherd Lara D LD

Applications in plant sciences 20170825 8

<h4>Premise of the study</h4>Microsatellite markers were developed for <i>Arthropodium cirratum</i> (Asparagaceae) to study population genetic structure and translocation of this species. These markers were tested for cross-amplification in two other <i>Arthropodium</i> species.<h4>Methods and results</h4>Sixteen microsatellite markers were developed from a genomic library and tested in three populations of <i>A. cirratum</i>. The loci exhibited one to five alleles per locus, with private allele ...[more]

PMID: 28924514

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