Dataset Information

Essential Role of the Cytoplasmic Chemoreceptor TlpT in the De Novo Formation of Chemosensory Complexes in Rhodobacter sphaeroides.

ABSTRACT: Bacterial chemosensory proteins form large hexagonal arrays. Several key features of chemotactic signaling depend on these large arrays, namely, cooperativity between receptors, sensitivity, integration of different signals, and adaptation. The best-studied arrays are the membrane-associated arrays found in most bacteria. Rhodobacter sphaeroides has two spatially distinct chemosensory arrays, one is transmembrane and the other is cytoplasmic. These two arrays work together to control a single flagellum. Deletion of one of the soluble chemoreceptors, TlpT, results in the loss of the formation of the cytoplasmic array. Here, we show the expression of TlpT in a tlpT deletion background results in the reformation of the cytoplasmic array. The number of arrays formed is dependent on the cell length, indicating spatial limitations on the number of arrays in a cell and stochastic assembly. Deletion of PpfA, a protein required for the positioning and segregation of the cytoplasmic array, results in slower array formation upon TlpT expression and fewer arrays, suggesting it accelerates cluster assembly.IMPORTANCE Bacterial chemosensory arrays are usually membrane associated and consist of thousands of copies of receptors, adaptor proteins, kinases, and adaptation enzymes packed into large hexagonal structures. Rhodobacter sphaeroides also has cytoplasmic arrays, which divide and segregate using a chromosome-associated ATPase, PpfA. The expression of the soluble chemoreceptor TlpT is shown to drive the formation of the arrays, accelerated by PpfA. The positioning of these de novo arrays suggests their position is the result of stochastic assembly rather than active positioning.

SUBMITTER: Jones CW

PROVIDER: S-EPMC5585706 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

Essential Role of the Cytoplasmic Chemoreceptor TlpT in the <i>De Novo</i> Formation of Chemosensory Complexes in Rhodobacter sphaeroides.

Jones Christopher W CW Armitage Judith P JP

Journal of bacteriology 20170905 19

Bacterial chemosensory proteins form large hexagonal arrays. Several key features of chemotactic signaling depend on these large arrays, namely, cooperativity between receptors, sensitivity, integration of different signals, and adaptation. The best-studied arrays are the membrane-associated arrays found in most bacteria. <i>Rhodobacter sphaeroides</i> has two spatially distinct chemosensory arrays, one is transmembrane and the other is cytoplasmic. These two arrays work together to control a si ...[more]

PMID: 28739674

Similar Datasets

Project description:The flagellar lipoprotein FlgP has been identified in several species of bacteria, and its absence provokes different phenotypes. In this study, we show that in the alphaproteobacterium Rhodobacter sphaeroides, a ΔflgP mutant is unable to assemble the hook and the filament. In contrast, the membrane/supramembrane (MS) ring and the flagellar rod appear to be assembled. In the absence of FlgP a severe defect in the transition from rod to hook polymerization occurs. In agreement with this idea, we noticed a reduction in the amount of intracellular flagellin and the chemotactic protein CheY4, both encoded by genes dependent on σ28 This suggests that in the absence of flgP the switch to export the anti-sigma factor, FlgM, does not occur. The presence of FlgP was detected by Western blot in samples of isolated wild-type filament basal bodies, indicating that FlgP is an integral part of the flagellar structure. In this regard, we show that FlgP interacts with FlgH and FlgT, indicating that FlgP should be localized closely to the L and H rings. We propose that FlgP could affect the architecture of the L ring, which has been recently identified to be responsible for the rod-hook transition.IMPORTANCE Flagellar based motility confers a selective advantage on bacteria by allowing migration to favorable environments or in pathogenic species to reach the optimal niche for colonization. The flagellar structure has been well established in Salmonella However, other accessory components have been identified in other species. Many of these have been implied in adapting the flagellar function to enable faster rotation, or higher torque. FlgP has been proposed to be the main component of the basal disk located underlying the outer membrane in Campylobacter jejuni and Vibrio fischeri Its role is still unclear, and its absence impacts motility differently in different species. The study of these new components will bring a better understanding of the evolution of this complex organelle.

Dataset Information

Essential Role of the Cytoplasmic Chemoreceptor TlpT in the De Novo Formation of Chemosensory Complexes in Rhodobacter sphaeroides.

Publications

Essential Role of the Cytoplasmic Chemoreceptor TlpT in the <i>De Novo</i> Formation of Chemosensory Complexes in Rhodobacter sphaeroides.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets