Ontology highlight
ABSTRACT:
SUBMITTER: Alderson TR
PROVIDER: S-EPMC5586491 | biostudies-literature | 2017 Aug
REPOSITORIES: biostudies-literature
Alderson T Reid TR Charlier Cyril C Torchia Dennis A DA Anfinrud Philip P Bax Ad A
Journal of the American Chemical Society 20170807 32
A method is introduced that permits direct observation of the rates at which backbone amide hydrogens become protected from solvent exchange after rapidly dropping the hydrostatic pressure inside the NMR sample cell from denaturing (2.5 kbar) to native (1 bar) conditions. The method is demonstrated for a pressure-sensitized ubiquitin variant that contains two Val to Ala mutations. Increased protection against hydrogen exchange with solvent is monitored as a function of time during the folding pr ...[more]