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In-line balanced detection stimulated Raman scattering microscopy.

ABSTRACT: We introduce a novel configuration for stimulated Raman scattering (SRS) microscopy, called In-line Balanced Detection (IBD), which employs a birefringent plate to generate a time-delayed polarization-multiplexed collinear replica of the probe, acting as a reference. Probe and reference cross the sample at the same position, thus maintaining their balance during image acquisition. IBD can be implemented in any conventional SRS setup, by adding a few simple elements, bringing its sensitivity close to the shot-noise limit even with a noisy laser. We tested IBD with a fiber-format laser system and observed signal-to-noise ratio improvement by up to 30?dB.

SUBMITTER: Crisafi F

PROVIDER: S-EPMC5587718 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature

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In-line balanced detection stimulated Raman scattering microscopy.

Crisafi Francesco F Kumar Vikas V Scopigno Tullio T Marangoni Marco M Cerullo Giulio G Polli Dario D

Scientific reports 20170906 1

We introduce a novel configuration for stimulated Raman scattering (SRS) microscopy, called In-line Balanced Detection (IBD), which employs a birefringent plate to generate a time-delayed polarization-multiplexed collinear replica of the probe, acting as a reference. Probe and reference cross the sample at the same position, thus maintaining their balance during image acquisition. IBD can be implemented in any conventional SRS setup, by adding a few simple elements, bringing its sensitivity clos ...[more]

PMID: 28878228

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