Unknown

Dataset Information

0

Microtubule-associated protein-4 controls nanovesicle dynamics and T cell activation.


ABSTRACT: The immune synapse (IS) is a specialized structure formed at the contact area between T lymphocytes and antigen-presenting cells (APCs) that is essential for the adaptive immune response. Proper T cell activation requires its polarization towards the APC, which is highly dependent on the tubulin cytoskeleton. Microtubule-associated protein-4 (MAP4) is a microtubule (MT)-stabilizing protein that controls MTs in physiological processes, such as cell division, migration, vesicular transport or primary cilia formation. In this study, we assessed the role of MAP4 in T cell activation. MAP4 decorates the pericentrosomal area and MTs of the T cell, and it is involved in MT detyrosination and stable assembly in response to T cell activation. In addition, MAP4 prompts the timely translocation of the MT-organizing center (MTOC) towards the IS and the dynamics of signaling nanovesicles that sustains T cell activation. However, MAP4 acts as a negative regulator of other T cell activation-related signals, including diacylglycerol (DAG) production and IL2 secretion. Our data indicate that MAP4 acts as a checkpoint molecule that balances positive and negative hallmarks of T cell activation.

SUBMITTER: Bustos-Moran E 

PROVIDER: S-EPMC5589067 | biostudies-literature | 2017 Apr

REPOSITORIES: biostudies-literature

altmetric image

Publications

Microtubule-associated protein-4 controls nanovesicle dynamics and T cell activation.

Bustos-Morán Eugenio E   Blas-Rus Noelia N   Martin-Cófreces Noa Beatriz NB   Sánchez-Madrid Francisco F  

Journal of cell science 20170216 7


The immune synapse (IS) is a specialized structure formed at the contact area between T lymphocytes and antigen-presenting cells (APCs) that is essential for the adaptive immune response. Proper T cell activation requires its polarization towards the APC, which is highly dependent on the tubulin cytoskeleton. Microtubule-associated protein-4 (MAP4) is a microtubule (MT)-stabilizing protein that controls MTs in physiological processes, such as cell division, migration, vesicular transport or prim  ...[more]

Similar Datasets

| S-EPMC5577222 | biostudies-literature
| S-EPMC7401817 | biostudies-literature
| S-EPMC3259197 | biostudies-literature
| S-EPMC3899817 | biostudies-literature
| S-EPMC2789662 | biostudies-literature
| S-EPMC6050278 | biostudies-literature
| S-EPMC7152617 | biostudies-literature
| S-EPMC8684730 | biostudies-literature
| S-EPMC3292199 | biostudies-literature
| S-EPMC6331727 | biostudies-literature