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Integrated Microfluidic System for Gene Silencing and Cell Migration.

ABSTRACT: Metastasis involves the phenotype transition of cancer cells to gain invasiveness, and the following migration at the tumor site. Here an integrated microfluidic chip to study this process is presented by combining on-chip delivery of siRNA for gene silencing and cell migration assay. The major advantage of the integrated chip is the simple input of cells and gene transfection materials, and the ultimate output of migration ability. The reverse-fishbone structure and 0.7× phosphate-buffered saline solution are the optimized parameters for improved delivery efficiency. Using the chip, it is validated that cofilin plays an essential role in regulating cancer cell migration. The integrated chip may provide a simple and effective platform for biologists to easily check the role of specific genes in metastasis.

SUBMITTER: Liu Z

PROVIDER: S-EPMC5589337 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature

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Integrated Microfluidic System for Gene Silencing and Cell Migration.

Liu Zongbin Z Han Xin X Zhou Qing Q Chen Rui R Fruge Shelby S Jo Myeong Chan MC Ma Yuan Y Li Ziyin Z Yokoi Kenji K Qin Lidong L

Advanced biosystems 20170518 6

Metastasis involves the phenotype transition of cancer cells to gain invasiveness, and the following migration at the tumor site. Here an integrated microfluidic chip to study this process is presented by combining on-chip delivery of siRNA for gene silencing and cell migration assay. The major advantage of the integrated chip is the simple input of cells and gene transfection materials, and the ultimate output of migration ability. The reverse-fishbone structure and 0.7× phosphate-buffered sali ...[more]

PMID: 28890929

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