Ontology highlight
ABSTRACT:
SUBMITTER: Toyofuku M
PROVIDER: S-EPMC5589764 | biostudies-literature | 2017 Sep
REPOSITORIES: biostudies-literature
Toyofuku Masanori M Cárcamo-Oyarce Gerardo G Yamamoto Tatsuya T Eisenstein Fabian F Hsiao Chien-Chi CC Kurosawa Masaharu M Gademann Karl K Pilhofer Martin M Nomura Nobuhiko N Eberl Leo L
Nature communications 20170907 1
Bacteria release membrane vesicles (MVs) that play important roles in various biological processes. However, the mechanisms of MV formation in Gram-positive bacteria are unclear, as these cells possess a single cytoplasmic membrane that is surrounded by a thick cell wall. Here we use live cell imaging and electron cryo-tomography to describe a mechanism for MV formation in Bacillus subtilis. We show that the expression of a prophage-encoded endolysin in a sub-population of cells generates holes ...[more]