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A method for estimating intracellular ion concentration using optical nanosensors and ratiometric imaging.

ABSTRACT: Optical nanoparticle (NP)-based sensors have been widely implemented as tools for detection of targeted ions and biomolecules. The NP sensing platform offer a modular design that can incorporate different sensing components for greater target specificity and the ability to tune the dynamic range, as well as encapsulation of multiple dyes to generate a ratiometric signal with varying spectra. Despite these advantages, demonstrating quantitative ion imaging for intracellular measurement still possess a major challenge. Here, we describe fundamentals that enable intracellular validation of this approach using ion-selective nanosensors for investigating calcium (Ca2+) as a model ion. While conventional indicators can improve individual aspects of indicator performance such as Kd, wavelength, and ratiometric measurements, the use of NP sensors can achieve combined benefits of addressing these issues simultaneously. The nanosensor incorporates highly calcium-selective ionophores and two fluorescence indicators that act as signal transducers to facilitate quantitative ratiometric imaging. For intracellular Ca2+ application, the sensors are fine-tuned to physiological sensing range, and live-cell imaging and quantification are demonstrated in HeLa cells loaded with nanosensors and their responsiveness to carbachol-evoked store release (~400?nM). The current nanosensor design thus provides a promising sensing platform for real-time detection and optical determination of intracellular ions.

SUBMITTER: Rong G

PROVIDER: S-EPMC5589868 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature

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A method for estimating intracellular ion concentration using optical nanosensors and ratiometric imaging.

Rong Guoxin G Kim Eric H EH Poskanzer Kira E KE Clark Heather A HA

Scientific reports 20170907 1

Optical nanoparticle (NP)-based sensors have been widely implemented as tools for detection of targeted ions and biomolecules. The NP sensing platform offer a modular design that can incorporate different sensing components for greater target specificity and the ability to tune the dynamic range, as well as encapsulation of multiple dyes to generate a ratiometric signal with varying spectra. Despite these advantages, demonstrating quantitative ion imaging for intracellular measurement still poss ...[more]

PMID: 28883429

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Dataset Information

A method for estimating intracellular ion concentration using optical nanosensors and ratiometric imaging.

Publications

A method for estimating intracellular ion concentration using optical nanosensors and ratiometric imaging.

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