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Tracking the tumor invasion front using long-term fluidic tumoroid culture.

ABSTRACT: The analysis of invading leader cells at the tumor invasion front is of significant interest as these cells may possess a coordinated functional and molecular phenotype which can be targeted for therapy. However, such analyses are currently limited by available technologies. Here, we report a fluidic device for long-term three-dimensional tumoroid culture which recapitulated the tumor invasion front, allowing for both quantification of invasive potential and molecular characterization of invasive leader cells. Preliminary analysis of the invasion front indicated an association with cell proliferation and higher expression of growth differentiation factor 15 (GDF15). This device makes real-time tracking of invading leader cell phenotypes possible and has potential for use with patient material for clinical risk stratification and personalized medicine.

SUBMITTER: Aw Yong KM

PROVIDER: S-EPMC5589910 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature

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Tracking the tumor invasion front using long-term fluidic tumoroid culture.

Aw Yong Koh Meng KM Li Zida Z Merajver Sofia D SD Fu Jianping J

Scientific reports 20170907 1

The analysis of invading leader cells at the tumor invasion front is of significant interest as these cells may possess a coordinated functional and molecular phenotype which can be targeted for therapy. However, such analyses are currently limited by available technologies. Here, we report a fluidic device for long-term three-dimensional tumoroid culture which recapitulated the tumor invasion front, allowing for both quantification of invasive potential and molecular characterization of invasiv ...[more]

PMID: 28883652

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