Unknown

Dataset Information

0

Early X chromosome inactivation during human preimplantation development revealed by single-cell RNA-sequencing.


ABSTRACT: In female mammals, one X chromosome is transcriptionally inactivated (XCI), leading to dosage compensation between sexes, fundamental for embryo viability. A previous study using single-cell RNA-sequencing (scRNA-seq) data proposed that female human preimplantation embryos achieve dosage compensation by downregulating both Xs, a phenomenon named dampening of X expression. Using a novel pipeline on those data, we identified a decrease in the proportion of biallelically expressed X-linked genes during development, consistent with XCI. Moreover, we show that while the expression sum of biallelically expressed X-linked genes decreases with embryonic development, their median expression remains constant, rejecting the hypothesis of X dampening. In addition, analyses of a different dataset of scRNA-seq suggest the appearance of X-linked monoallelic expression by the late blastocyst stage in females, another hallmark of initiation of XCI. Finally, we addressed the issue of dosage compensation between the single active X and autosomes in males and females for the first time during human preimplantation development, showing emergence of X to autosome dosage compensation by the upregulation of the active X chromosome in both male and female embryonic stem cells. Our results show compelling evidence of an early process of X chromosome inactivation during human preimplantation development.

SUBMITTER: Moreira de Mello JC 

PROVIDER: S-EPMC5589911 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature

altmetric image

Publications

Early X chromosome inactivation during human preimplantation development revealed by single-cell RNA-sequencing.

Moreira de Mello Joana C JC   Fernandes Gustavo R GR   Vibranovski Maria D MD   Pereira Lygia V LV  

Scientific reports 20170907 1


In female mammals, one X chromosome is transcriptionally inactivated (XCI), leading to dosage compensation between sexes, fundamental for embryo viability. A previous study using single-cell RNA-sequencing (scRNA-seq) data proposed that female human preimplantation embryos achieve dosage compensation by downregulating both Xs, a phenomenon named dampening of X expression. Using a novel pipeline on those data, we identified a decrease in the proportion of biallelically expressed X-linked genes du  ...[more]

Similar Datasets

| S-EPMC8100148 | biostudies-literature
| S-EPMC9454834 | biostudies-literature
| S-EPMC10277609 | biostudies-literature
| S-EPMC2694969 | biostudies-literature
| S-EPMC9845752 | biostudies-literature
| S-EPMC6704272 | biostudies-literature
| S-EPMC9346283 | biostudies-literature
| S-EPMC7502599 | biostudies-literature
| S-EPMC4950944 | biostudies-literature
| S-EPMC9061815 | biostudies-literature