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Targeted anion transporter delivery by coiled-coil driven membrane fusion.

ABSTRACT: Synthetic anion transporters (anionophores) have potential as biomedical research tools and therapeutics. However, the efficient and specific delivery of these highly lipophilic molecules to a target cell membrane is non-trivial. Here, we investigate the delivery of a powerful anionophore to artificial and cell membranes using a coiled-coil-based delivery system inspired by SNARE membrane fusion proteins. Incorporation of complementary lipopeptides into the lipid membranes of liposomes and cell-sized giant unilamellar vesicles (GUVs) facilitated the delivery of a powerful anionophore into GUVs, where its anion transport activity was monitored in real time by fluorescence microscopy. Similar results were achieved using live cells engineered to express a halide-sensitive fluorophore. We conclude that coiled-coil driven membrane fusion is a highly efficient system to deliver anionophores to target cell membranes.

SUBMITTER: Mora NL

PROVIDER: S-EPMC5592372 | biostudies-literature | 2016 Mar

REPOSITORIES: biostudies-literature

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Targeted anion transporter delivery by coiled-coil driven membrane fusion.

Mora Nestor Lopez NL Bahreman Azadeh A Valkenier Hennie H Li Hongyu H Sharp Thomas H TH Sheppard David N DN Davis Anthony P AP Kros Alexander A

Chemical science 20160107 3

Synthetic anion transporters (anionophores) have potential as biomedical research tools and therapeutics. However, the efficient and specific delivery of these highly lipophilic molecules to a target cell membrane is non-trivial. Here, we investigate the delivery of a powerful anionophore to artificial and cell membranes using a coiled-coil-based delivery system inspired by SNARE membrane fusion proteins. Incorporation of complementary lipopeptides into the lipid membranes of liposomes and cell- ...[more]

PMID: 28936326

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