The L-type Voltage-Gated Calcium Channel co-localizes with Syntaxin 1A in nano-clusters at the plasma membrane.

ABSTRACT: The secretory signal elicited by membrane depolarization traverses from the Ca²⁺-bound α₁1.2 pore-forming subunit of the L-type Ca²⁺-channel (Cav1.2) to syntaxin 1 A (Sx1A) via an intra-membrane signaling mechanism. Here, we report the use of two-color Photo-Activated-Localization-Microscopy (PALM) to determine the relation between Cav1.2 and Sx1A in single-molecule detail. We observed nanoscale co-clusters of PAmCherry-tagged Sx1A and Dronpa-tagged α₁1.2 at a ~1:1 ratio. PAmCherry-tagged Sx1A^C145A, or PAmCherry-tagged Sx2, an inactive Cav1.2 modulator, in which Cys145 is a Ser residue, showed no co-clustering. These results are  consistent with the crucial role of the single cytosolic Sx1ACys145 in clustering with Cav1.2. Cav1.2 and t

SUBMITTER: Sajman J 

PROVIDER: S-EPMC5595989 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature