Ontology highlight
ABSTRACT:
SUBMITTER: Hamada H
PROVIDER: S-EPMC5597576 | biostudies-literature | 2017 Sep
REPOSITORIES: biostudies-literature
Hamada Haruyasu H Linghu Qianyan Q Nagira Yozo Y Miki Ryuji R Taoka Naoaki N Imai Ryozo R
Scientific reports 20170913 1
The currently favoured method for wheat (Triticum aestivum L.) transformation is inapplicable to many elite cultivars because it requires callus culture and regeneration. Here, we developed a simple, reproducible, in planta wheat transformation method using biolistic DNA delivery without callus culture or regeneration. Shoot apical meristems (SAMs) grown from dry imbibed seeds were exposed under a microscope and subjected to bombardment with different-sized gold particles coated with the GFP gen ...[more]