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Mitochondrial toxicity of triclosan on mammalian cells.

ABSTRACT: Effects of triclosan (5-chloro-2'-(2,4-dichlorophenoxy)phenol) on mammalian cells were investigated using human peripheral blood mono nuclear cells (PBMC), keratinocytes (HaCaT), porcine spermatozoa and kidney tubular epithelial cells (PK-15), murine pancreatic islets (MIN-6) and neuroblastoma cells (MNA) as targets. We show that triclosan (1-10 ?g ml^-1) depolarised the mitochondria, upshifted the rate of glucose consumption in PMBC, HaCaT, PK-15 and MNA, and subsequently induced metabolic acidosis. Triclosan induced a regression of insulin producing pancreatic islets into tiny pycnotic cells and necrotic death. Short exposure to low concentrations of triclosan (30 min, ?1 ?g/ml) paralyzed the high amplitude tail beating and progressive motility of spermatozoa, within 30 min exposure, depolarized the spermatozoan mitochondria and hyperpolarised the acrosome region of the sperm head and the flagellar fibrous sheath (distal part of the flagellum). Experiments with isolated rat liver mitochondria showed that triclosan impaired oxidative phosphorylation, downshifted ATP synthesis, uncoupled respiration and provoked excessive oxygen uptake. These exposure concentrations are 100-1000 fold lower that those permitted in consumer goods. The mitochondriotoxic mechanism of triclosan differs from that of valinomycin, cereulide and the enniatins by not involving potassium ionophoric activity.

SUBMITTER: Ajao C

PROVIDER: S-EPMC5598359 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature

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Mitochondrial toxicity of triclosan on mammalian cells.

Ajao Charmaine C Andersson Maria A MA Teplova Vera V VV Nagy Szabolcs S Gahmberg Carl G CG Andersson Leif C LC Hautaniemi Maria M Kakasi Balazs B Roivainen Merja M Salkinoja-Salonen Mirja M

Toxicology reports 20150407

Effects of triclosan (5-chloro-2'-(2,4-dichlorophenoxy)phenol) on mammalian cells were investigated using human peripheral blood mono nuclear cells (PBMC), keratinocytes (HaCaT), porcine spermatozoa and kidney tubular epithelial cells (PK-15), murine pancreatic islets (MIN-6) and neuroblastoma cells (MNA) as targets. We show that triclosan (1-10 μg ml<sup>-1</sup>) depolarised the mitochondria, upshifted the rate of glucose consumption in PMBC, HaCaT, PK-15 and MNA, and subsequently induced meta ...[more]

PMID: 28962398

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Mitochondrial toxicity of triclosan on mammalian cells.

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Mitochondrial toxicity of triclosan on mammalian cells.

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