Ontology highlight
ABSTRACT:
SUBMITTER: Yoshinari S
PROVIDER: S-EPMC5599603 | biostudies-literature | 2017 Sep
REPOSITORIES: biostudies-literature
Scientific reports 20170914 1
Methanothermobacter thermoautotrophicus RNA ligase (MthRnl) catalyzes formation of phosphodiester bonds between the 5'-phosphate and 3'-hydroxyl termini of single-stranded RNAs. It can also react with RNA with a 3'-phosphate end to generate a 2',3'-cyclic phosphate. Here, we show that MthRnl can additionally remove adenosine from the 3'-terminus of the RNA to produce 3'-deadenylated RNA, RNA(3'-rA). This 3'-deadenylation activity is metal-dependent and requires a 2'-hydroxyl at both the terminal ...[more]