CD44 induced enhancement of phosphatase activity and calcium influx: Modifications of EGR-1 expression and cell proliferation.

ABSTRACT: The purpose of this study was to investigate how CD44 impaired Akt phosphorylation, EGR-1 expression and cell proliferation. E6.1 Jurkat cells, which lack endogenous CD44 expression, were engineered to express CD44. Previously we showed that Akt is hypophosphorylated, EGR-1 expression is reduced and proliferation is impaired in CD44 expressing E6.1 Jurkat cells. The cell cycle was studied using flow cytometry and the role of calcium (Ca²⁺) in Akt phosphorylation and EGR-1 expression was investigated using Western blotting. Phosphatase activity was assessed using a commercially available kit. CD44 expressing cells showed disruption at the G₁ to S transition. Chelation of Ca²⁺ from the culture media impaired Akt phosphorylation and EGR-1 expression in both CD

SUBMITTER: Racine RR 

PROVIDER: S-EPMC5600419 | biostudies-literature | 2016 Jul

REPOSITORIES: biostudies-literature