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Value Assignment of Vitamin D Metabolites in Vitamin D Standardization Program Serum Samples.

ABSTRACT: Assay variability has been cited as an obstacle to establishing optimal vitamin D exposure. As part of the Vitamin D Standardization Program (VDSP) effort to standardize the measurement of total 25-hydroxyvitamin D [25(OH)D], the value assignment of total 25(OH)D in 50 single-donor serum samples was performed using two isotope-dilution LC with tandem MS methods. Both methods are recognized as reference measurement procedures (RMPs) by the Joint Committee for Traceability in Laboratory Medicine. These samples and their assigned values serve as the foundation for several aspects of the VDSP. To our knowledge, this is the first time that two RMPs have been used to assign 25(OH)D values to such a large number of serum samples.

SUBMITTER: Phinney KW

PROVIDER: S-EPMC5601309 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature

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Value Assignment of Vitamin D Metabolites in Vitamin D Standardization Program Serum Samples.

Phinney Karen W KW Camara Johanna E JE Tai Susan S-C SS Sander Lane C LC Wise Stephen A SA De Grande Linde A C LAC Thienpont Linda M LM Possolo Antonio M AM Toman Blaza B Sempos Christopher T CT Betz Joseph M JM Coates Paul M PM

Journal of AOAC International 20170616 5

Assay variability has been cited as an obstacle to establishing optimal vitamin D exposure. As part of the Vitamin D Standardization Program (VDSP) effort to standardize the measurement of total 25-hydroxyvitamin D [25(OH)D], the value assignment of total 25(OH)D in 50 single-donor serum samples was performed using two isotope-dilution LC with tandem MS methods. Both methods are recognized as reference measurement procedures (RMPs) by the Joint Committee for Traceability in Laboratory Medicine. ...[more]

PMID: 28623654

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