Ontology highlight
ABSTRACT:
SUBMITTER: Steringer JP
PROVIDER: S-EPMC5601999 | biostudies-literature | 2017 Jul
REPOSITORIES: biostudies-literature
eLife 20170719
FGF2 is secreted from cells by an unconventional secretory pathway. This process is mediated by direct translocation across the plasma membrane. Here, we define the minimal molecular machinery required for FGF2 membrane translocation in a fully reconstituted inside-out vesicle system. FGF2 membrane translocation is thermodynamically driven by PI(4,5)P<sub>2</sub>-induced membrane insertion of FGF2 oligomers. The latter serve as dynamic translocation intermediates of FGF2 with a subunit number in ...[more]