An enzymatic strategy to asymmetrically branched N-glycans.
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ABSTRACT:
SUBMITTER: Calderon AD
PROVIDER: S-EPMC5603075 | biostudies-literature | 2017 Sep
REPOSITORIES: biostudies-literature
Publications
An enzymatic strategy to asymmetrically branched N-glycans.
Calderon Angie D AD Zhou Jun J Guan Wanyi W Wu Zhigang Z Guo Yuxi Y Bai Jing J Li Qing Q Wang Peng George PG Fang Junqiang J Li Lei L
Organic & biomolecular chemistry 20170901 35
An enzymatic strategy was developed to generate asymmetrically branched N-glycans from natural sources by using a panel of glycosidases and glycosyltransferases. Briefly, LacZ β-galactosidase was employed to selectively trim symmetrically branched N-glycans isolated from bovine fetuin. The yielding structures were then converted to asymmetrically branched core structures by robust glycosyltransferase for further extension. ...[more]
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