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Cytokine/chemokine expression associated with Human Pegivirus (HPgV) infection in women with HIV.

ABSTRACT: A beneficial impact of the Human Pegivirus (HPgV)-formerly called GB virus C (GBV-C)-on HIV disease progression has been reported previously. One possible mechanism by which HPgV inhibits HIV replication is an alteration of the cytokine/chemokine milieu. Their expression has not been specifically evaluated in women despite their influence on disease progression and the possibility of gender-based differences in expression. Moreover, the impact of HPgV genotype on cytokine/chemokine expression is unknown. Sera levels of IL-2, IL-4, IL-7, IL-8, IL-10, IL-12p70, IL-13, IFN?, TNF?, IP-10, MIP-1?, MIP-1?, and TGF-?1 were quantified in 150 HIV-positive women based on HPgV RNA status. Cytokines/chemokines with detection rates of at least 50% included IL-2, IL-4, IL-8, IL-10, IL-12p70, IFN?, TNF?, IP-10, MIP-1?, MIP-1?, and TGF-?1 . Absolute values were significantly higher for HPgV positive compared to HPgV negative women for IL-7, IL-13, IL-12p70, and IFN?. Absolute values were significantly lower for HPgV positive women for IL-4, IL-8, TGF-?1 , and IP-10. IFN? values were higher for HPgV genotype 2 than for genotype 1 (P?=?0.036). Further study of cytokine/chemokine regulation by HPgV may ultimately lead to the development of novel therapeutic agents to treat HIV infection and/or the design of vaccine strategies that mimic the "protective" effects of HPgV replication.

SUBMITTER: Blackard JT

PROVIDER: S-EPMC5603382 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature

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Cytokine/chemokine expression associated with Human Pegivirus (HPgV) infection in women with HIV.

Blackard Jason T JT Ma Gang G Welge Jeffrey A JA Taylor Lynn E LE Mayer Kenneth H KH Klein Robert S RS Celentano David D DD Sobel Jack D JD Jamieson Denise J DJ King Caroline C CC

Journal of medical virology 20170828 11

A beneficial impact of the Human Pegivirus (HPgV)-formerly called GB virus C (GBV-C)-on HIV disease progression has been reported previously. One possible mechanism by which HPgV inhibits HIV replication is an alteration of the cytokine/chemokine milieu. Their expression has not been specifically evaluated in women despite their influence on disease progression and the possibility of gender-based differences in expression. Moreover, the impact of HPgV genotype on cytokine/chemokine expression is ...[more]

PMID: 28460153

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Project description:BackgroundHuman Pegivirus (HPgV) may have a beneficial effect on HIV disease progression in co-infected patients; however, the virologic characteristics of this infection are not well defined. In this study, we determined HPgV viremia prevalence in Mexico and provide new insights to understand HPgV infection and HPgV/HIV co-infection.MethodsWe analyzed and quantified 7,890 serum samples for HPgV viremia by One-Step RT-Real-Time PCR, 6,484 from healthy blood donors and 1,406 from HIV-infected patients. Data on HIV progression were obtained from patients' records. HPgV genotyping was performed in 445 samples by nested PCR of the 5'URT region. Finite Mixture Models were used to identify clustering patterns of HPgV viremia in blood donors and co-infected antiretroviral (ART)-naïve patients.ResultsHPgV was detected in 2.98% of blood donors and 33% of HIV patients, with a wide range of viral loads. The most prevalent genotypes were 3 (58.6%)and 2 (33.7%). HPgV viral loads from healthy blood donors and HPgV/HIV+ ART-naïve co-infected patients were clustered into two component distributions, low and high, with a cut-off point of 5.07log10 and 5.06log10, respectively. High HPgV viremia was associated with improved surrogate markers of HIV infection, independent of the estimated duration of HIV infection or HIV treatment.ConclusionsHPgV prevalence in Mexico was similar to that reported for other countries. The prevalent genotypes could be related to Mexico's geographic location and ethnicity, since genotype 2 is frequent in the United States and Europe and genotype 3 in Asia and Amerindian populations. HPgV viral load demonstrated two patterns of replication, low and high. The more pronounced beneficial response observed in co-infected patients with high HPgV viremia may explain discrepancies found between other studies. Mechanisms explaining high and low HPgV replication should be explored to determine whether the persistently elevated replication depends on host or viral factors.

Dataset Information

Cytokine/chemokine expression associated with Human Pegivirus (HPgV) infection in women with HIV.

Publications

Cytokine/chemokine expression associated with Human Pegivirus (HPgV) infection in women with HIV.

Similar Datasets

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