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Orange, red, yellow: biosynthesis of azaphilone pigments in Monascus fungi.

ABSTRACT: Monascus azaphilone pigments (MonAzPs) are very widely used as food colorants, but their biosynthetic pathway has remained poorly characterized for more than half a century. In this study, the individual steps of MonAzPs biosynthesis in Monascus ruber M7 were elucidated by a combination of targeted gene knockouts, heterologous gene expression, and in vitro chemical and enzymatic reactions. This study describes the first rational engineering of MonAzPs biosynthesis and provides a roadmap for future pathway engineering efforts directed towards the selective production of the most valuable pigments and serves as a model for the biosynthesis of fungal azaphilones in general.

SUBMITTER: Chen W

PROVIDER: S-EPMC5603960 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature

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Orange, red, yellow: biosynthesis of azaphilone pigments in Monascus fungi.

Chen Wanping W Chen Runfa R Liu Qingpei Q He Yi Y He Kun K Ding Xiaoli X Kang Lijing L Guo Xiaoxiao X Xie Nana N Zhou Youxiang Y Lu Yuanyuan Y Cox Russell J RJ Molnár István I Li Mu M Shao Yanchun Y Chen Fusheng F

Chemical science 20170424 7

Monascus azaphilone pigments (MonAzPs) are very widely used as food colorants, but their biosynthetic pathway has remained poorly characterized for more than half a century. In this study, the individual steps of MonAzPs biosynthesis in Monascus ruber M7 were elucidated by a combination of targeted gene knockouts, heterologous gene expression, and in vitro chemical and enzymatic reactions. This study describes the first rational engineering of MonAzPs biosynthesis and provid ...[more]

PMID: 28959415

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