Dataset Information

First Genome Sequence of Shallot Latent Carlavirus from Allium macrostemon Bunge.

ABSTRACT: A wild Japanese garlic plant (Allium macrostemon Bunge, wild onion) with leaves showing chlorotic stripes was collected in Saitama Prefecture, Japan. Genome sequencing showed that it was infected with shallot latent carlavirus. The genomic sequence of this virus is reported for the first time from wild onion.

SUBMITTER: Ohshima K

PROVIDER: S-EPMC5604777 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature

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First Genome Sequence of Shallot Latent Carlavirus from <i>Allium macrostemon</i> Bunge.

Ohshima Kazusato K Okamura Kouta K Yasaka Ryosuke R Fukuda Shinji S Ishimaru Kanji K Tomitaka Yasuhiro Y Yamashita Kazuo K

Genome announcements 20170817 33

A wild Japanese garlic plant (<i>Allium macrostemon</i> Bunge, wild onion) with leaves showing chlorotic stripes was collected in Saitama Prefecture, Japan. Genome sequencing showed that it was infected with shallot latent carlavirus. The genomic sequence of this virus is reported for the first time from wild onion. ...[more]

PMID: 28818904

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Project description:BackgroundAllii Macrostemonis Bulbus is also named Xiebai in China. It is an edible vegetable, and also a famous herb for treating coronary heart disease. Allium chinense G. Don (ACGD) and Allium macrostemon Bunge (AMB) are it botanical sources. The aim of this study was to explore the cardioprotective effects, and decipher the visual spatial distribution and absolute content of primary metabolites derived from these two herbs.MethodsH9c2 cells were used to perform the hypoxia-reoxygenation (H/R)-induced myocardial injury model. Their protective effects were evaluated by apoptosis levels. Furthermore, matrix-assisted laser desorption/ionization time-of-flight tandem mass spectrometry imaging approach (MALDI-TOF MSI) was carried out to present the spatial location of primary metabolites including fatty acids, amino acids, carotenoids, and vitamins in these two Allium herbs. Multiple analytical methods were applied to perform quantitative analysis of these primary metabolites in AMB and ACGD bulbs by liquid chromatography tandem mass spectrometry (LC-MS).ResultsFirst, AMB and ACGD extracts both could increase the cell viability in H9c2 cells, and attenuate H/R-induced injury. They markedly decreased apoptosis, accompanied by activating the BCL-2/BAX pathway. Further, MALDI-TOF MSI-based relative quantification results showed several amino acids, fatty acids, carotenoids, and vitamins were largely rich in the tunics and outside scales of fresh bulbs, while some primary metabolites were abundant in their developing flower buds. Absolute quantification results displayed total contents of amino acids in ACGD bulbs were higher than those in AMB, while total contents of fatty acids and vitamins provides opposite trends in these two Allium herbs. The total contents of carotenoids and trace elements showed no significant differences between AMB and ACGD samples.ConclusionsThis study would be helpful to understand the myocardial injury protection effects of these two Allium herbs, and the spatial accumulation and quantitative content levels of their main nutrients.

Dataset Information

First Genome Sequence of Shallot Latent Carlavirus from Allium macrostemon Bunge.

Publications

First Genome Sequence of Shallot Latent Carlavirus from <i>Allium macrostemon</i> Bunge.

Similar Datasets

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