Flux Analysis of Free Amino Sugars and Amino Acids in Soils by Isotope Tracing with a Novel Liquid Chromatography/High Resolution Mass Spectrometry Platform.

ABSTRACT: Soil fluxomics analysis can provide pivotal information for understanding soil biochemical pathways and their regulation, but direct measurement methods are rare. Here, we describe an approach to measure soil extracellular metabolite (amino sugar and amino acid) concentrations and fluxes based on a ¹⁵N isotope pool dilution technique via liquid chromatography and high-resolution mass spectrometry. We produced commercially unavailable ¹⁵N and ¹³C labeled amino sugars and amino acids by hydrolyzing peptidoglycan isolated from isotopically labeled bacterial biomass and used them as tracers (¹⁵N) and internal standards (¹³C). High-resolution (Orbitrap Exactive) MS with a resolution of 50 000 allowed us to separate different stable isotope

SUBMITTER: Hu Y 

PROVIDER: S-EPMC5605124 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature