Unknown

Dataset Information

0

Extracellular HSP60 triggers tissue regeneration and wound healing by regulating inflammation and cell proliferation.


ABSTRACT: After injury, zebrafish can restore many tissues that do not regenerate well in mammals, making it a useful vertebrate model for studying regenerative biology. We performed a systematic screen to identify genes essential for hair cell regeneration in zebrafish, and found that the heat shock protein Hspd1 (Hsp60) has a critical role in the regeneration of hair cells and amputated caudal fins. We showed HSP60-injected extracellularly promoted cell proliferation and regeneration in both hair cells and caudal fins. We showed that hspd1 mutant was deficient in leukocyte infiltration at the site of injury. Topical application of HSP60 in a diabetic mouse skin wound model dramatically accelerated wound healing compared with controls. Stimulation of human peripheral blood mononuclear cells with HSP60 triggered a specific induction of M2 phase CD163-positive monocytes. Our results demonstrate that the normally intracellular chaperonin HSP60 has an extracellular signalling function in injury inflammation and tissue regeneration, likely through promoting the M2 phase for macrophages.

SUBMITTER: Pei W 

PROVIDER: S-EPMC5605149 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature

altmetric image

Publications

Extracellular HSP60 triggers tissue regeneration and wound healing by regulating inflammation and cell proliferation.

Pei Wuhong W   Tanaka Katsuya K   Huang Sunny C SC   Xu Lisha L   Liu Baoying B   Sinclair Jason J   Idol Jennifer J   Varshney Gaurav K GK   Huang Haigen H   Lin Shuo S   Nussenblatt Robert B RB   Mori Ryoichi R   Burgess Shawn M SM  

NPJ Regenerative medicine 20161027


After injury, zebrafish can restore many tissues that do not regenerate well in mammals, making it a useful vertebrate model for studying regenerative biology. We performed a systematic screen to identify genes essential for hair cell regeneration in zebrafish, and found that the heat shock protein Hspd1 (Hsp60) has a critical role in the regeneration of hair cells and amputated caudal fins. We showed HSP60-injected extracellularly promoted cell proliferation and regeneration in both hair cells  ...[more]

Similar Datasets

| S-EPMC11292631 | biostudies-literature
| S-EPMC11468066 | biostudies-literature
| S-EPMC10861198 | biostudies-literature
| S-EPMC4563743 | biostudies-literature
| S-EPMC11342954 | biostudies-literature
| S-EPMC10876488 | biostudies-literature
| S-EPMC10381614 | biostudies-literature
| S-EPMC5831781 | biostudies-literature
| S-EPMC5156902 | biostudies-literature
| S-EPMC7519026 | biostudies-literature