Ontology highlight
ABSTRACT:
SUBMITTER: Kastilan R
PROVIDER: S-EPMC5607246 | biostudies-literature | 2017 Sep
REPOSITORIES: biostudies-literature
Kastilan Robin R Boes Alexander A Spiegel Holger H Voepel Nadja N Chudobová Ivana I Hellwig Stephan S Buyel Johannes Felix JF Reimann Andreas A Fischer Rainer R
Scientific reports 20170920 1
Pichia pastoris is a simple and powerful expression platform that has the ability to produce a wide variety of recombinant proteins, ranging from simple peptides to complex membrane proteins. A well-established fermentation strategy is available comprising three main phases: a batch phase, followed by a glycerol fed-batch phase that increases cell density, and finally an induction phase for product expression using methanol as the inducer. We previously used this three-phase strategy at the 15-L ...[more]