Origins of the enhanced affinity of RNA-protein interactions triggered by RNA phosphorodithioate backbone modification.
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ABSTRACT:
SUBMITTER: Yang X
PROVIDER: S-EPMC5608642 | biostudies-literature | 2017 Sep
REPOSITORIES: biostudies-literature
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Origins of the enhanced affinity of RNA-protein interactions triggered by RNA phosphorodithioate backbone modification.
Yang Xianbin X Dinuka Abeydeera N N Liu Feng-Wu FW Egli Martin M
Chemical communications (Cambridge, England) 20170901 76
The well-characterized interaction between the MS2 coat protein and its cognate RNA hairpin was used to evaluate changes in affinity as a result of phosphorodithioate (PS2) replacing phosphate by biolayer interferometry (BLI). A structure-based analysis of the data provides insights into the origins of the enhanced affinity of RNA-protein interactions triggered by the PS2 moiety. ...[more]
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