Ontology highlight
ABSTRACT:
SUBMITTER: Dean KM
PROVIDER: S-EPMC5609504 | biostudies-literature | 2017 Feb
REPOSITORIES: biostudies-literature
Optica 20170201 2
In fluorescence microscopy, the serial acquisition of 2D images to form a 3D volume limits the maximum imaging speed. This is particularly evident when imaging adherent cells in a light-sheet fluorescence microscopy format, as their elongated morphologies require ~200 image planes per image volume. Here, by illuminating the specimen with three light-sheets, each independently detected, we present a light-efficient, crosstalk free, and volumetrically parallelized 3D microscopy technique that is o ...[more]