Identification and characterisation of lamprey protein kinase C delta-like gene.

ABSTRACT: Protein kinase C-? (PKC-?), a member of the lipid-regulated serine/threonine PKC family, has been implicated in a wide range of important cellular processes, such as cell growth, differentiation, and apoptosis. Lampreys belong to the most primitive class of vertebrates, and there is little information on PKC-? in these animals. In this study, a PKC-?-like cDNA sequence and deduced PKC-?-like amino acid sequence were identified in the Japanese lamprey (Lampetra japonica). The PKC-?-like gene shared approximately 60% sequence identity with its homologs in jawed vertebrates. The anti-PKC-?-like polyclonal antibodies were well prepared, and experiments showed that PKC-?-like was primarily distributed in the supraneural body of the lamprey. Both mRNA and protein levels of PKC-?-like in supraneural body cells were increased after incubation with cis-diaminedichloroplatinum (CDDP). Moreover, PKC-?-like protein induced the apoptosis of HEK-293T cells. In addition, the activation of PKC-?-like resulted in apoptosis. Conversely, the inhibition of PKC-?-like activity disrupted the CDDP-mediated induction of cellular apoptosis. These results indicate that PKC-?-like identified in lampreys might play an important role in apoptosis in jawless vertebrates.

SUBMITTER: Xu Y 

PROVIDER: S-EPMC5610172 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature