Dataset Information

Gene Expression Profiling in Human Lung Cells Exposed to Isoprene-Derived Secondary Organic Aerosol.

ABSTRACT: Secondary organic aerosol (SOA) derived from the photochemical oxidation of isoprene contributes a substantial mass fraction to atmospheric fine particulate matter (PM_2.5). The formation of isoprene SOA is influenced largely by anthropogenic emissions through multiphase chemistry of its multigenerational oxidation products. Considering the abundance of isoprene SOA in the troposphere, understanding mechanisms of adverse health effects through inhalation exposure is critical to mitigating its potential impact on public health. In this study, we assessed the effects of isoprene SOA on gene expression in human airway epithelial cells (BEAS-2B) through an air-liquid interface exposure. Gene expression profiling of 84 oxidative stress and 249 inflammation-associated human genes was performed. Our results show that the expression levels of 29 genes were significantly altered upon isoprene SOA exposure under noncytotoxic conditions (p < 0.05), with the majority (22/29) of genes passing a false discovery rate threshold of 0.3. The most significantly affected genes belong to the nuclear factor (erythroid-derived 2)-like 2 (Nrf2) transcription factor network. The Nrf2 function is confirmed through a reporter cell line. Together with detailed characterization of SOA constituents, this study reveals the impact of isoprene SOA exposure on lung responses and highlights the importance of further understanding its potential health outcomes.

SUBMITTER: Lin YH

PROVIDER: S-EPMC5610912 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature

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Gene Expression Profiling in Human Lung Cells Exposed to Isoprene-Derived Secondary Organic Aerosol.

Lin Ying-Hsuan YH Arashiro Maiko M Clapp Phillip W PW Cui Tianqu T Sexton Kenneth G KG Vizuete William W Gold Avram A Jaspers Ilona I Fry Rebecca C RC Surratt Jason D JD

Environmental science & technology 20170705 14

Secondary organic aerosol (SOA) derived from the photochemical oxidation of isoprene contributes a substantial mass fraction to atmospheric fine particulate matter (PM<sub>2.5</sub>). The formation of isoprene SOA is influenced largely by anthropogenic emissions through multiphase chemistry of its multigenerational oxidation products. Considering the abundance of isoprene SOA in the troposphere, understanding mechanisms of adverse health effects through inhalation exposure is critical to mitigat ...[more]

PMID: 28636383

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Project description:The nitrate radical (NO3) oxidation of isoprene is an important contributor to secondary organic aerosol (SOA). Isoprene has two double bonds which allow for multigeneration oxidation to occur. The effects of multigeneration chemistry on the gas- and particle-phase product distributions of the isoprene + NO3 system are not fully understood. In this study, we conduct chamber experiments by varying the ratio of N2O5 (precursor of NO3) to isoprene concentration from 1:1 to 14:1 to investigate the formation of products in both phases under different oxidation levels. Multigeneration chemistry leads to the formation of gas-phase products which then partition into particle phase depending on the product volatility; first-generation products (15-36% of total SOA) such as C5H9NO5 and C10H16N2O9 have volatility (log10C* = 1.0-3.0 using the partitioning method and log10C* = 2.6-4.5 using the formula method) 1-5 orders of magnitude higher than second-generation products (37-57% of total SOA, log10C* = -0.8-2.1 using the partitioning method and log10C* = -3.7-1.8 using the formula method) such as C5H8,10N2O8, C5H9N3O10, and C10H17N3O13. The fast reaction rate constants of first-generation products (estimated to be on the order of 10-13 cm3 molecules-1 s-1 at 295 K) and the lower volatility of second-generation products result in increased SOA yields when NO3 availability increases and multigeneration chemistry is enhanced. Specifically, an increase of up to 300% in SOA yield is observed when the N2O5/isoprene ratio increases from 1:1 to 3:1; from 5.7% (organic aerosol mass concentration, ΔM o = 4.2 μg/m3) to 16.3% (ΔM o = 11.9 μg/m3) when the reacted isoprene concentration is 25 ppb and from 3.1% (ΔM o = 1.2 μg/m3) to 12.4% (ΔM o = 5.4 μg/m3) when the reacted isoprene concentration is 15 ppb. The maximum SOA yield occurs when the N2O5/isoprene ratio is greater than or equal to 3:1 as a combined result of multigeneration chemistry and peroxy radicals (RO2) fate. We encourage future studies to consider both factors, which can vary under different laboratory and ambient conditions, when comparing SOA yields to better understand any differences observed. Our results highlight that multigeneration chemistry and the updated parameters including reaction rate constants and volatility distribution of products should be considered to enable a more comprehensive representation and prediction of SOA formation from NO3 oxidation of isoprene in atmospheric models.

Dataset Information

Gene Expression Profiling in Human Lung Cells Exposed to Isoprene-Derived Secondary Organic Aerosol.

Publications

Gene Expression Profiling in Human Lung Cells Exposed to Isoprene-Derived Secondary Organic Aerosol.

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