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Integrating running water monitoring tools with the Micro Biological Survey (MBS) method to improve water quality assessment.

ABSTRACT: Running water habitats are among the most altered aquatic systems by human activities driving an increase in the organic components and the associated bacterial load as well. To contribute in improving the monitoring activities in running waters, here we tested the validity of the new Micro Biological Survey (MBS) method to specifically assess the bacterial load in running waters focusing on Total Viable Counts (at 22°C and 37°C) and Escherichia coli (at 44°C) in order to propose a new prognostic tool for watercourses. MBS method is an alternative colorimetric method for counting bacterial load in water and food samples that is easy to use and leads to a reliable and simple interpretation of results, being also faster and less expensive than traditional methods. Then, we compared MBS with the traditionally used reference method for the bacterial load, and with the most used biotic index for Italian watercourses based on the benthic invertebrates: the Extended Biotic Index (EBI). The last comparison was performed to validate the use of MBS in biomonitoring activities since the benthic invertebrate multi-species assemblage (and then EBI) alter own structure mainly depending on the organic component variation. During the first part of the study, the assessment of both linearity (regressions among bacterial concentrations) and accuracy (significant correlation between a measured value and a value used as reference) confirmed the validity of the MBS method. Second, the linear regressions between the three investigated microbial parameters vs. both physical-chemical descriptors and EBI, revealed the usefulness of MBS as a valid tool for routine microbiological analyses involved in rapid and easy field monitoring activities. This represents the first attempt to evaluate the river microbial status by exploiting the innovative MBS on running waters to propose it as new valuable monitoring tool in the biomonitoring field.

SUBMITTER: Traversetti L

PROVIDER: S-EPMC5612684 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature

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Integrating running water monitoring tools with the Micro Biological Survey (MBS) method to improve water quality assessment.

Traversetti Lorenzo L Losito Francesca F Arienzo Alyexandra A Stalio Ottavia O Antonini Giovanni G Scalici Massimiliano M

PloS one 20170925 9

Running water habitats are among the most altered aquatic systems by human activities driving an increase in the organic components and the associated bacterial load as well. To contribute in improving the monitoring activities in running waters, here we tested the validity of the new Micro Biological Survey (MBS) method to specifically assess the bacterial load in running waters focusing on Total Viable Counts (at 22°C and 37°C) and Escherichia coli (at 44°C) in order to propose a new prognosti ...[more]

PMID: 28945808

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Project description:BACKGROUND:Adequate supply of safe drinking-water remains a critical issue in most developing countries. The whole western region of Cameroon doesn't have a sustainable continuous water supply system, which leads most people to use potentially contaminated water sources to meet their daily water needs. Previous, studies carried out in similar areas of Cameroon have highlighted the poor bacteriological quality of water sources used as drinking-water by the local populations. METHODS:This study used the Micro Biological Survey method, a rapid colorimetric test for the quantitative detection of Coliforms in water samples. 22 water sources (12 improved and 10 unimproved) were identified; 1 water sample of 50?ml was collected in sterile plastic tubes, immediately kept in a refrigerator box and transported to the laboratory for analysis. 1?ml of each sample was inoculated in the Coliforms Micro Biological Survey (Coli MBS) vials initially rehydrated with 10?ml of sterile distilled water. The Coli MBS vials were closed, shaken for about 30?s for homogenization and then incubated at 37?°C. From the initial red color of the Coli MBS vials, changes in color of the reaction vials were monitored at three different time intervals (12?h, 19?h and 24?h), corresponding to three levels of contamination. RESULTS:The average distance (8.7?m) of the latrines from the nearest water source was less than the minimal recommended distance (15?m) to ovoid external contamination. The pH of water samples ranged from 5.5 to 8.3 and the maximum temperature found (26?°C) was almost at level favorable to outbreaks of waterborne diseases such as cholera. The presence of Total Coliforms was detected in 90.91% of the samples. 40% of samples were positive 12?h after the analysis beginning. High level of contamination was observed in unimproved water sources, 50% after 12?h corresponding to Total Coliforms concentration of 10?<?x?<?103?CFU/ml and the other samples after 19?h (Total Coliforms concentration: 1?<?x?<?10?CFU/ml). CONCLUSION:This study revealed the poor microbiological quality of water used by local populations of our study sites. There is need to conduct further qualitative microbiology studies to isolate potential germs involved in outcome of diarrheal diseases.

Dataset Information

Integrating running water monitoring tools with the Micro Biological Survey (MBS) method to improve water quality assessment.

Publications

Integrating running water monitoring tools with the Micro Biological Survey (MBS) method to improve water quality assessment.

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