Methods to generate genetically engineered mouse models of soft tissue sarcoma.
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ABSTRACT:
SUBMITTER: Dodd RD
PROVIDER: S-EPMC5612825 | biostudies-literature | 2015
REPOSITORIES: biostudies-literature
Publications
Methods to generate genetically engineered mouse models of soft tissue sarcoma.
Dodd Rebecca D RD Añó Leonor L Blum Jordan M JM Li Zhizhong Z Van Mater David D Kirsch David G DG
Methods in molecular biology (Clifton, N.J.) 20150101
We discuss the generation of primary soft tissue sarcomas in mice using the Cre-loxP system to activate conditional mutations in oncogenic Kras and the tumor suppressor p53 (LSL-Kras(G12D/+); p53(flox/flox)). Sarcomas can be generated either by adenoviral delivery of Cre recombinase, activation of transgenic Cre recombinase with tamoxifen, or through transplantation of isolated satellite cells with Cre activation in vitro. Various applications of these models are discussed, including anticancer ...[more]
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