Dataset Information

Two genomes of highly polyphagous lepidopteran pests (Spodoptera frugiperda, Noctuidae) with different host-plant ranges.

ABSTRACT: Emergence of polyphagous herbivorous insects entails significant adaptation to recognize, detoxify and digest a variety of host-plants. Despite of its biological and practical importance - since insects eat 20% of crops - no exhaustive analysis of gene repertoires required for adaptations in generalist insect herbivores has previously been performed. The noctuid moth Spodoptera frugiperda ranks as one of the world's worst agricultural pests. This insect is polyphagous while the majority of other lepidopteran herbivores are specialist. It consists of two morphologically indistinguishable strains ("C" and "R") that have different host plant ranges. To describe the evolutionary mechanisms that both enable the emergence of polyphagous herbivory and lead to the shift in the host preference, we analyzed whole genome sequences from laboratory and natural populations of both strains. We observed huge expansions of genes associated with chemosensation and detoxification compared with specialist Lepidoptera. These expansions are largely due to tandem duplication, a possible adaptation mechanism enabling polyphagy. Individuals from natural C and R populations show significant genomic differentiation. We found signatures of positive selection in genes involved in chemoreception, detoxification and digestion, and copy number variation in the two latter gene families, suggesting an adaptive role for structural variation.

SUBMITTER: Gouin A

PROVIDER: S-EPMC5613006 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature

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Two genomes of highly polyphagous lepidopteran pests (Spodoptera frugiperda, Noctuidae) with different host-plant ranges.

Gouin Anaïs A Bretaudeau Anthony A Nam Kiwoong K Gimenez Sylvie S Aury Jean-Marc JM Duvic Bernard B Hilliou Frédérique F Durand Nicolas N Montagné Nicolas N Darboux Isabelle I Kuwar Suyog S Chertemps Thomas T Siaussat David D Bretschneider Anne A Moné Yves Y Ahn Seung-Joon SJ Hänniger Sabine S Grenet Anne-Sophie Gosselin AG Neunemann David D Maumus Florian F Luyten Isabelle I Labadie Karine K Xu Wei W Koutroumpa Fotini F Escoubas Jean-Michel JM Llopis Angel A Maïbèche-Coisne Martine M Salasc Fanny F Tomar Archana A Anderson Alisha R AR Khan Sher Afzal SA Dumas Pascaline P Orsucci Marion M Guy Julie J Belser Caroline C Alberti Adriana A Noel Benjamin B Couloux Arnaud A Mercier Jonathan J Nidelet Sabine S Dubois Emeric E Liu Nai-Yong NY Boulogne Isabelle I Mirabeau Olivier O Le Goff Gaelle G Gordon Karl K Oakeshott John J Consoli Fernando L FL Volkoff Anne-Nathalie AN Fescemyer Howard W HW Marden James H JH Luthe Dawn S DS Herrero Salvador S Heckel David G DG Wincker Patrick P Kergoat Gael J GJ Amselem Joelle J Quesneville Hadi H Groot Astrid T AT Jacquin-Joly Emmanuelle E Nègre Nicolas N Lemaitre Claire C Legeai Fabrice F d'Alençon Emmanuelle E Fournier Philippe P

Scientific reports 20170925 1

Emergence of polyphagous herbivorous insects entails significant adaptation to recognize, detoxify and digest a variety of host-plants. Despite of its biological and practical importance - since insects eat 20% of crops - no exhaustive analysis of gene repertoires required for adaptations in generalist insect herbivores has previously been performed. The noctuid moth Spodoptera frugiperda ranks as one of the world's worst agricultural pests. This insect is polyphagous while the majority of other ...[more]

PMID: 28947760

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Project description:Simple Summary Mythimna separata and Spodoptera frugiperda are two destructive pests worldwide. In this study, for the first time, we evaluated their sterol metabolic capacity. The results showed that Spodoptera frugiperda required less sterol for normal growth, which helped them survive better when cholesterol was unavailable. Both insects consistently showed high fitness when they fed on cholestanol. Cholestanone enabled most individuals of S. frugiperda to pupate but caused remarkable lethality to M. separata at their early developmental stage. Comparative studies indicated that S. frugiperda was more efficient in converting ketone into available stanol than M. separata. Therefore, they perform differently in terms of their sterol demand and metabolism although these two species are closely related. The divergences in sterol nutritional biology between the two closely related insect species reflect adaptive and evolutive changes in sterol metabolism, which may help us to better understand the potential of using phytosterol-manipulated plants to control pests. Abstract Insects are sterol auxotrophs and typically obtain sterols from food. However, the sterol demand and metabolic capacity vary greatly among species, even for closely related species. The low survival of many insects on atypical sterols, such as cholestanol and cholestanone, raises the possibility of using sterol-modified plants to control insect herbivore pests. In this study, we evaluated two devastating migratory crop pests, Mythimna separata and Spodoptera frugiperda, in response to atypical sterols and explored the reasons that caused the divergences in sterol nutritional biology between them. Contrary to M. separata, S. frugiperda had unexpectedly high survival on cholestanone, and nearly 80% of the individuals pupated. Comparative studies, including insect response to multiple diets and larval body sterol/steroids analysis, were performed to explain their differences in cholestanone usage. Our results showed that, in comparison to M. separata, the superiority of S. frugiperda on cholestanone can be attributed to its higher efficiency of converting ketone into available stanol and its lower demand for sterols, which resulted in a better survival when cholesterol was unavailable. This research will help us to better understand insect sterol nutritional biology and the potential of using atypical sterols to control herbivorous insect pests.

Project description:Intra- and interspecific interactions within communities of species that utilize the same resources are characterized by competition or facilitation. The noctuid stemborers, Busseola fusca and Sesamia calamistis, and the crambid stemborer, Chilo partellus were the most important pests of maize in sub-Saharan Africa before the recent "invasion" of fall armyworm (FAW), Spodoptera frugiperda, which currently seriously limits maize yields in Africa. This new pest is interacting with the stemborer community at the larval stage in the use of maize resources. From a work done by Ntiri et al. [13] on the influence of temperature on the larval intra- and interspecific resources utilization within the community of Lepidoptera stemborers involving B. fusca, S. calamistis, and C. partellus, there is a need to update this study by adding the new pest, S. frugiperda, in order to understand the effect of temperature on the larval interactions of all these four species under the context of climate change. The influence of temperature on intra- and interspecific larval interactions was studied in the same protocol of Ntiri et al. [13] using artificial stems kept at different constant temperatures (15 °C, 20 °C, 25 °C, and 30 °C) in an incubator and assessing survival and relative growth rates of each species in single and multi-species experiments. After the inclusion of FAW into the experiments, with regard to relative growth rates, both intra- and interspecific competition was observed among all four species. With regard to survival rates, cannibalism can also explain the intra- and interspecific interactions observed among all four species. Interspecific competition was stronger between the stemborers than between the FAW and the stemborers. Similar to lepidopteran stemborers, temperature affected both survival and relative growth rates of the FAW as well. Regardless of the temperature, C. partellus was superior in interspecific interactions shown by higher relative growth and survival rates. The results suggest that the FAW will co-exist with stemborer species along entire temperature gradient, though competition and/or cannibalism with them is weak. In addition, temperature increases caused by climate change is likely to confer an advantage to C. partellus over the fall armyworm and the other noctuids.

Dataset Information

Two genomes of highly polyphagous lepidopteran pests (Spodoptera frugiperda, Noctuidae) with different host-plant ranges.

Publications

Two genomes of highly polyphagous lepidopteran pests (Spodoptera frugiperda, Noctuidae) with different host-plant ranges.

Similar Datasets

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