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Establishment of combined analytical method to extract the genes of interest from transcriptome data.


ABSTRACT: Techniques for analyzing genome-wide expression profiles, such as the microarray technique and next-generation sequencers, have been developed. While these techniques can provide a lot of information about gene expression, selection of genes of interest is complicated because of excessive gene expression data. Thus, many researchers use statistical methods or fold change as screening tools for finding gene sets whose expression is altered between groups, which may result in the loss of important information. In the present study, we aimed to establish a combined method for selecting genes of interest with a small magnitude of alteration in gene expression by coupling with proteome analysis. We used hypercholesterolemic rats to examine the effects of a crude herbal drug on gene expression and proteome profiles. We could not select genes of interest by using standard methods. However, by coupling with proteome analysis, we found several effects of the crude herbal drug on gene expression. Our results suggest that this method would be useful in selecting gene sets with expressions that do not show a large magnitude of alteration.

SUBMITTER: Ishihara A 

PROVIDER: S-EPMC5613277 | biostudies-literature | 2016 Sep

REPOSITORIES: biostudies-literature

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Establishment of combined analytical method to extract the genes of interest from transcriptome data.

Ishihara Akinori A   Yamanaka Hidenori H   Takahashi Reiko R   Numajiri Tomomi T   Kaneko Saki S   Ishizawa Yoko Y   Koya Sakuji S   Yamauchi Kiyoshi K  

Biochemistry and biophysics reports 20160519


Techniques for analyzing genome-wide expression profiles, such as the microarray technique and next-generation sequencers, have been developed. While these techniques can provide a lot of information about gene expression, selection of genes of interest is complicated because of excessive gene expression data. Thus, many researchers use statistical methods or fold change as screening tools for finding gene sets whose expression is altered between groups, which may result in the loss of important  ...[more]

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