Ontology highlight
ABSTRACT:
SUBMITTER: Picart-Palmade L
PROVIDER: S-EPMC5613304 | biostudies-literature | 2016 Sep
REPOSITORIES: biostudies-literature
Biochemistry and biophysics reports 20160602
The structure and stability of the fluorescent protein monomeric Kusabira Orange (mKO), a GFP-like protein, was studied under different pressure levels and in different chemical environments. At different pH values (between pH 7.4 and pH 4.0) and under a pressure up to 600 MPa (at 25 °C), mKO did not show significant fluorescence spectral changes, indicating a structural stability of the protein. In more extreme chemical conditions (at pH 4.0 in the presence of 0.8 M guanidine hydrochloride), a ...[more]