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Preparation and partial characterization of monoclonal antibodies specific for the nascent non-triple helical form of the type IV collagen alpha 1 chain.

ABSTRACT: This report describes the preparation and partial characterization of monoclonal antibodies that are reactive specifically with the nascently produced non-triple helical form of the type IV collagen ?1 chain, designated as NTH ?1(IV). These antibodies were nonreactive with the ?1 chain of the type IV collagen in the triple-helical conformation. Three antibodies, #141, #179 and #370, with different epitopes in NTH ?1(IV) were found to be reactive with the nascent polypeptide secreted from human normal cells and a human carcinoma cell line. The antibodies with different epitopes may provide a key method for elucidating the physiological function and tissue distribution of NTH ?1(IV), which is distinct from the chain derived from triple-helical type IV collagen.

SUBMITTER: Morita M

PROVIDER: S-EPMC5614550 | biostudies-literature | 2017 Mar

REPOSITORIES: biostudies-literature

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Preparation and partial characterization of monoclonal antibodies specific for the nascent non-triple helical form of the type IV collagen alpha 1 chain.

Morita Makoto M Sugihara Hidemitsu H Tokunaka Kazuhiro K Tomura Arihiro A Saiga Kan K Sato Takamichi T Imamura Yasutada Y Hayashi Toshihiko T

Biochemistry and biophysics reports 20161208

This report describes the preparation and partial characterization of monoclonal antibodies that are reactive specifically with the nascently produced non-triple helical form of the type IV collagen α1 chain, designated as NTH α1(IV). These antibodies were nonreactive with the α1 chain of the type IV collagen in the triple-helical conformation. Three antibodies, #141, #179 and #370, with different epitopes in NTH α1(IV) were found to be reactive with the nascent polypeptide secreted from human n ...[more]

PMID: 28955997

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