Dataset Information

Using computer simulation models to investigate the most promising microRNAs to improve muscle regeneration during ageing.

ABSTRACT: MicroRNAs (miRNAs) regulate gene expression through interactions with target sites within mRNAs, leading to enhanced degradation of the mRNA or inhibition of translation. Skeletal muscle expresses many different miRNAs with important roles in adulthood myogenesis (regeneration) and myofibre hypertrophy and atrophy, processes associated with muscle ageing. However, the large number of miRNAs and their targets mean that a complex network of pathways exists, making it difficult to predict the effect of selected miRNAs on age-related muscle wasting. Computational modelling has the potential to aid this process as it is possible to combine models of individual miRNA:target interactions to form an integrated network. As yet, no models of these interactions in muscle exist. We created the first model of miRNA:target interactions in myogenesis based on experimental evidence of individual miRNAs which were next validated and used to make testable predictions. Our model confirms that miRNAs regulate key interactions during myogenesis and can act by promoting the switch between quiescent/proliferating/differentiating myoblasts and by maintaining the differentiation process. We propose that a threshold level of miR-1 acts in the initial switch to differentiation, with miR-181 keeping the switch on and miR-378 maintaining the differentiation and miR-143 inhibiting myogenesis.

SUBMITTER: Proctor CJ

PROVIDER: S-EPMC5614911 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature

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Using computer simulation models to investigate the most promising microRNAs to improve muscle regeneration during ageing.

Proctor Carole J CJ Goljanek-Whysall Katarzyna K

Scientific reports 20170926 1

MicroRNAs (miRNAs) regulate gene expression through interactions with target sites within mRNAs, leading to enhanced degradation of the mRNA or inhibition of translation. Skeletal muscle expresses many different miRNAs with important roles in adulthood myogenesis (regeneration) and myofibre hypertrophy and atrophy, processes associated with muscle ageing. However, the large number of miRNAs and their targets mean that a complex network of pathways exists, making it difficult to predict the effec ...[more]

PMID: 28951568

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Dataset Information

Using computer simulation models to investigate the most promising microRNAs to improve muscle regeneration during ageing.

Publications

Using computer simulation models to investigate the most promising microRNAs to improve muscle regeneration during ageing.

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