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Overexpression of stathmin plays a pivotal role in the metastasis of esophageal squamous cell carcinoma.


ABSTRACT:

Purpose

Esophageal squamous cell carcinoma (ESCC) is a serious malignant tumor that affects human health. We analyzed the correlation between serum stathmin level and ESCC and elucidated the molecular mechanisms of stathmin's promotion of ESCC cell invasion and metastasis.

Methods

Stathmin level in ESCC and healthy control serum were detected by enzyme-linked immunosorbent assay (ELISA), and the clinical parameters were analyzed. We established ESCC cells with stathmin overexpression or knockdown and then evaluated the effects of stathmin on invasion and metastasis in ESCC. Differentially expressed genes were analyzed by Human Transcriptome Array and confirmed by RT-PCR. The expression levels of the integrin family, focal adhesion kinase (FAK) and extracellular signal-regulated kinase (ERK) were detected by immunoblotting.

Results

Serum levels of stathmin were significantly higher in ESCC than in control serum and associated with lymph node metastasis, tumor stage and size. Furthermore, we found that stathmin promoted migration and invasion of ESCC cells in vitro and in vivo. In addition, we confirmed that the activation of the integrin?5?1/FAK/ERK pathway is increased in stathmin-overexpression cells and accelerates cell motility by enhancing cell adhesion ability.

Conclusion

Stathmin may predict a potential metastasis biomarker for ESCC.

SUBMITTER: Han G 

PROVIDER: S-EPMC5617461 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature

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Publications

Overexpression of stathmin plays a pivotal role in the metastasis of esophageal squamous cell carcinoma.

Han Gaijing G   Wu Zongyong Z   Zhao Nan N   Zhou Lanping L   Liu Fang F   Niu Fangfei F   Xu Yang Y   Zhao Xiaohang X  

Oncotarget 20170627 37


<h4>Purpose</h4>Esophageal squamous cell carcinoma (ESCC) is a serious malignant tumor that affects human health. We analyzed the correlation between serum stathmin level and ESCC and elucidated the molecular mechanisms of stathmin's promotion of ESCC cell invasion and metastasis.<h4>Methods</h4>Stathmin level in ESCC and healthy control serum were detected by enzyme-linked immunosorbent assay (ELISA), and the clinical parameters were analyzed. We established ESCC cells with stathmin overexpress  ...[more]

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