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Functionalized AIE nanoparticles with efficient deep-red emission, mitochondrial specificity, cancer cell selectivity and multiphoton susceptibility.

ABSTRACT: Multiphoton microscopy is an exciting tool for biomedical research because it can be used to image single cells in vivo due to its greater penetration depth, lower phototoxicity and higher resolution when compared to confocal laser scanning microscopy. This helps researchers understand how certain cells change over time and evaluate the efficacy of different therapies. Herein, we report a new AIE luminogen (AIEgen), abbreviated as TPE-TETRAD, with a favorable absorption and efficient deep-red emission in the solid state. TPE-TETRAD possesses a high two-photon absorption cross-section (313 MG at 830 nm) and a rich array of non-linear optical properties including aggregation-induced three-photon luminescence. Biotinylated TPE-TETRAD nanoparticles are also fabricated and applied to stain mitochondria in live cancer cells with high specificity. The purpose of this study is to characterize a novel deep-red AIEgen and fabricate biotinylated nanoparticles for applications as (1) biocompatible and photostable AIE probes for specific mitochondria imaging and (2) multiphoton imaging probes suitable for two/three-photon fluorescence microscopy.

SUBMITTER: Nicol A

PROVIDER: S-EPMC5618339 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature

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Functionalized AIE nanoparticles with efficient deep-red emission, mitochondrial specificity, cancer cell selectivity and multiphoton susceptibility.

Nicol Alexander A Qin Wei W Kwok Ryan T K RTK Burkhartsmeyer Jeffrey Mark JM Zhu Zhenfeng Z Su Huifang H Luo Wenwen W Lam Jacky W Y JWY Qian Jun J Wong Kam Sing KS Tang Ben Zhong BZ

Chemical science 20170509 6

Multiphoton microscopy is an exciting tool for biomedical research because it can be used to image single cells <i>in vivo</i> due to its greater penetration depth, lower phototoxicity and higher resolution when compared to confocal laser scanning microscopy. This helps researchers understand how certain cells change over time and evaluate the efficacy of different therapies. Herein, we report a new AIE luminogen (AIEgen), abbreviated as TPE-TETRAD, with a favorable absorption and efficient deep ...[more]

PMID: 28970884

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Project description:Aggregation induced emission (AIE)-active bright two-photon fluorescent probes with second near-infrared (NIR-II) light excitability can be used for efficient brain bioimaging studies, wherein the fabrication of water-dispersible nanoparticles by encapsulating the hydrophobic probes with amphiphilic polymer holds the key to ensuring biocompatibility and in vivo adaptability. However, barely any study has evaluated the structural requirements that can substantially affect the water-dispersible nanoparticle formation ability of an organic AIE-active dye with amphiphilic polymers. The present study systematically assessed the structural dependency of a well-known acrylonitrile based AIE system/fluorogenic core upon the formation of water-dispersible nanoparticles and elucidated how the structural modifications can impact the in vivo two-photon imaging. Methods: A total of four acrylonitrile-based aggregation induced emission (AIE)-active two-photon (TP) fluorescent probes (AIETP, AIETP C1, AIETP C2 and AIETP C3) have been judiciously designed and synthesized with structural variations to realize how the structural alterations could substantially influence the water-dispersible nanoparticle formation ability (with amphiphilic polymers) and photo-stability to impact the in vivo imaging. Results: It has been found that the incorporation of the phenyl-thiazole unit in AIETP, AIETP C2 and AIETP C3 facilitated the formation of water-dispersible nanoparticles (NPs) with amphiphilic polymers (Pluronic F127) whereas the presence of only phenyl moiety instead in AIETP C1 could not meet the suitable condition to form the NPs with good aqueous dispersibility. Rationally designed AIETP NPs that exhibited higher brightness, improved photostability and good two-photon absorption cross section was successfully employed for in vivo brain vasculature imaging. Conclusions: Robust noninvasive 2D and 3D two-photon (NIR-II light, 1040 nm) brain vasculature imaging with beneficial attributes such as outstanding penetration depth (800 µm) and exceptional spatial resolution (1.92 µm), were achieved by utilizing AIETP NPs in this study.

Dataset Information

Functionalized AIE nanoparticles with efficient deep-red emission, mitochondrial specificity, cancer cell selectivity and multiphoton susceptibility.

Publications

Functionalized AIE nanoparticles with efficient deep-red emission, mitochondrial specificity, cancer cell selectivity and multiphoton susceptibility.

Similar Datasets

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