Dataset Information
A cerebrospinal fluid microRNA signature as biomarker for glioblastoma.
Ontology highlight
ABSTRACT: Purpose
To develop a cerebrospinal fluid (CSF) miRNA diagnostic biomarker for glioblastoma.Experimental design
Glioblastoma tissue and matched CSF from the same patient (obtained prior to tumor manipulation) were profiled by TaqMan OpenArray® Human MicroRNA Panel. CSF miRNA profiles from glioblastoma patients and controls were created from three discovery cohorts and confirmed in two validation cohorts.Results
miRNA profiles from clinical CSF correlated with those found in glioblastoma tissues. Comparison of CSF miRNA profiles between glioblastoma patients and non-brain tumor patients yielded a tumor "signature" consisting of nine miRNAs. The "signature" correlated with glioblastoma tumor volume (p=0.008). When prospectively applied to cisternal CSF, the sensitivity and specificity of the 'signature' for glioblastoma detection were 67% and 80%, respectively. For lumbar CSF, the sensitivity and specificity of the signature were 28% and 95%, respectively. Comparable results were obtained from analyses of CSF extracellular vesicles (EVs) and crude CSF.Conclusion
We report a CSF miRNA signature as a "liquid biopsy" diagnostic platform for glioblastoma.
SUBMITTER: Akers JC
PROVIDER: S-EPMC5620295 | biostudies-literature | 2017 Sep
REPOSITORIES: biostudies-literature
Publications
A cerebrospinal fluid microRNA signature as biomarker for glioblastoma.
Oncotarget 20170601 40
<h4>Purpose</h4>To develop a cerebrospinal fluid (CSF) miRNA diagnostic biomarker for glioblastoma.<h4>Experimental design</h4>Glioblastoma tissue and matched CSF from the same patient (obtained prior to tumor manipulation) were profiled by TaqMan OpenArray<sup>®</sup> Human MicroRNA Panel. CSF miRNA profiles from glioblastoma patients and controls were created from three discovery cohorts and confirmed in two validation cohorts.<h4>Results</h4>miRNA profiles from clinical CSF correlated with th ...[more]
