Dataset Information

Carbapenemase VCC-1-Producing Vibrio cholerae in Coastal Waters of Germany.

ABSTRACT: During antimicrobial drug resistance testing for Vibrio spp. from coastal waters of Germany, we identified 4 nontoxigenic, carbapenem-resistant V. cholerae isolates. We used whole-genome sequencing to identify the carbapenemase gene bla_VCC-1. In addition, a molecular survey showed that more bla_VCC-1-harboring isolates are present in coastal waters of Germany.

SUBMITTER: Hammerl JA

PROVIDER: S-EPMC5621562 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature

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Carbapenemase VCC-1-Producing Vibrio cholerae in Coastal Waters of Germany.

Hammerl Jens A JA Jäckel Claudia C Bortolaia Valeria V Schwartz Keike K Bier Nadja N Hendriksen Rene S RS Guerra Beatriz B Strauch Eckhard E

Emerging infectious diseases 20171001 10

During antimicrobial drug resistance testing for Vibrio spp. from coastal waters of Germany, we identified 4 nontoxigenic, carbapenem-resistant V. cholerae isolates. We used whole-genome sequencing to identify the carbapenemase gene bla<sub>VCC-1</sub>. In addition, a molecular survey showed that more bla<sub>VCC-1</sub>-harboring isolates are present in coastal waters of Germany. ...[more]

PMID: 28930017

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Project description:BackgroundOuter membrane vesicles (OMVs) released from Gram-negative bacteria can serve as vehicles for the translocation of virulence factors. Vibrio cholerae produce OMVs but their putative role in translocation of effectors involved in pathogenesis has not been well elucidated. The V. cholerae cytolysin (VCC), is a pore-forming toxin that lyses target eukaryotic cells by forming transmembrane oligomeric β-barrel channels. It is considered a potent toxin that contributes to V. cholerae pathogenesis. The mechanisms involved in the secretion and delivery of the VCC have not been extensively studied.Methodology/principal findingsOMVs from V. cholerae strains were isolated and purified using a differential centrifugation procedure and Optiprep centrifugation. The ultrastructure and the contents of OMVs were examined under the electron microscope and by immunoblot analyses respectively. We demonstrated that VCC from V. cholerae strain V:5/04 was secreted in association with OMVs and the release of VCC via OMVs is a common feature among V. cholerae strains. The biological activity of OMV-associated VCC was investigated using contact hemolytic assay and epithelial cell cytotoxicity test. It showed toxic activity on both red blood cells and epithelial cells. Our results indicate that the OMVs architecture might play a role in stability of VCC and thereby can enhance its biological activities in comparison with the free secreted VCC. Furthermore, we tested the role of OMV-associated VCC in host cell autophagy signalling using confocal microscopy and immunoblot analysis. We observed that OMV-associated VCC triggered an autophagy response in the target cell and our findings demonstrated for the first time that autophagy may operate as a cellular defence mechanism against an OMV-associated bacterial virulence factor.Conclusion/significanceBiological assays of OMVs from the V. cholerae strain V:5/04 demonstrated that OMV-associated VCC is indeed biologically active and induces toxicity on mammalian cells and furthermore can induce autophagy.

Dataset Information

Carbapenemase VCC-1-Producing Vibrio cholerae in Coastal Waters of Germany.

Publications

Carbapenemase VCC-1-Producing Vibrio cholerae in Coastal Waters of Germany.

Similar Datasets

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