Dataset Information
?V?3 Integrin regulates astrocyte reactivity.
Ontology highlight
ABSTRACT: Background
Neuroinflammation involves cytokine release, astrocyte reactivity and migration. Neuronal Thy-1 promotes DITNC1 astrocyte migration by engaging ?V?3 Integrin and Syndecan-4. Primary astrocytes express low levels of these receptors and are unresponsive to Thy-1; thus, inflammation and astrocyte reactivity might be necessary for Thy-1-induced responses.Methods
Wild-type rat astrocytes (TNF-activated) or from human SOD1G93A transgenic mice (a neurodegenerative disease model) were used to evaluate cell migration, Thy-1 receptor levels, signaling molecules, and reactivity markers.Results
Thy-1 induced astrocyte migration only after TNF priming. Increased expression of ?V?3 Integrin, Syndecan-4, P2X7R, Pannexin-1, Connexin-43, GFAP, and iNOS were observed in TNF-treated astrocytes. Silencing of ?3 Integrin prior to TNF treatment prevented Thy-1-induced migration, while ?3 Integrin over-expression was sufficient to induce astrocyte reactivity and allow Thy-1-induced migration. Finally, hSOD1G93A astrocytes behave as TNF-treated astrocytes since they were reactive and responsive to Thy-1.Conclusions
Therefore, inflammation induces expression of ?V?3 Integrin and other proteins, astrocyte reactivity, and Thy-1 responsiveness. Importantly, ectopic control of ?3 Integrin levels modulates these responses regardless of inflammation.
SUBMITTER: Lagos-Cabre R
PROVIDER: S-EPMC5622429 | biostudies-literature | 2017 Sep
REPOSITORIES: biostudies-literature
Publications
α<sub>V</sub>β<sub>3</sub> Integrin regulates astrocyte reactivity.
Journal of neuroinflammation 20170929 1
<h4>Background</h4>Neuroinflammation involves cytokine release, astrocyte reactivity and migration. Neuronal Thy-1 promotes DITNC1 astrocyte migration by engaging α<sub>V</sub>β<sub>3</sub> Integrin and Syndecan-4. Primary astrocytes express low levels of these receptors and are unresponsive to Thy-1; thus, inflammation and astrocyte reactivity might be necessary for Thy-1-induced responses.<h4>Methods</h4>Wild-type rat astrocytes (TNF-activated) or from human SOD1<sup>G93A</sup> transgenic mice ...[more]
