Ontology highlight
ABSTRACT:
SUBMITTER: Shin D
PROVIDER: S-EPMC5624781 | biostudies-literature | 2017 Oct
REPOSITORIES: biostudies-literature
Shin Donghyuk D Na Wooju W Lee Ji-Hyung JH Kim Gyuhee G Baek Jiseok J Park Seok Hee SH Choi Cheol Yong CY Lee Sangho S
eLife 20171002
Rab GTPases, which are involved in intracellular trafficking pathways, have recently been reported to be ubiquitinated. However, the functions of ubiquitinated Rab proteins remain unexplored. Here we show that Rab5 is monoubiquitinated on K116, K140, and K165. Upon co-transfection with ubiquitin, Rab5 exhibited abnormalities in endosomal localization and EGF-induced EGF receptor degradation. Rab5 K140R and K165R mutants restored these abnormalities, whereas K116R did not. We derived structural m ...[more]