Dataset Information

Non-conventional role of haemoglobin beta in breast malignancy.

ABSTRACT:

Background

Besides its role as oxygen transporter, recent findings suggest that haemoglobin beta (HBB) may have roles in other contexts.

Methods

We evaluated the impact of HBB expression in primary human breast cancers, and in breast cancer cell lines overexpressing HBB by in vitro and in vivo studies. Publicly available microarray databases were used to perform multivariate survival analyses.

Results

A significantly higher expression of HBB was observed in invasive carcinoma histotypes vs in situ counterparts, along with a positive correlation between HBB and the Ki67 proliferation marker. HBB-overexpressing breast cancer cells migrate and invade more, show HIF-1? upregulation and their conditioned media enhances angiogenesis. Blocking the oxygen-binding site of HBB reverts the increase of migration and HIF-1? upregulation observed in HBB-overexpressing breast cancer cells. Orthotopically implanted MDA-MB-231 overexpressing HBB (MDA-HBB) generated tumours with faster growth rate and increased neoangiogenesis. Moreover, local recurrence and visceral metastases were observed only in MDA-HBB-implanted mice. Similar results were observed with 4T1 mouse breast cancer cells. Finally, bioinformatics analyses of public data sets correlated high HBB expression with lower overall survival.

Conclusions

HBB expression increases breast cancer cells aggressiveness and associates with poor prognosis, pointing to HBB as a novel biomarker for breast cancer progression.

SUBMITTER: Ponzetti M

PROVIDER: S-EPMC5625664 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature

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Publications

Non-conventional role of haemoglobin beta in breast malignancy.

Ponzetti Marco M Capulli Mattia M Angelucci Adriano A Ventura Luca L Monache Simona Delle SD Mercurio Cinzia C Calgani Alessia A Sanità Patrizia P Teti Anna A Rucci Nadia N

British journal of cancer 20170803 7

<h4>Background</h4>Besides its role as oxygen transporter, recent findings suggest that haemoglobin beta (HBB) may have roles in other contexts.<h4>Methods</h4>We evaluated the impact of HBB expression in primary human breast cancers, and in breast cancer cell lines overexpressing HBB by in vitro and in vivo studies. Publicly available microarray databases were used to perform multivariate survival analyses.<h4>Results</h4>A significantly higher expression of HBB was observed in invasive carcino ...[more]

PMID: 28772282

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Dataset Information

Non-conventional role of haemoglobin beta in breast malignancy.

Background

Methods

Results

Conclusions

Publications

Non-conventional role of haemoglobin beta in breast malignancy.

Similar Datasets

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