Ontology highlight
ABSTRACT: Background
The direct comparison of CA19.9, circulating tumour cells (CTCs) and circulating tumour DNA (ctDNA) using endoscopic ultrasound-guided fine needle aspiration (EUS-FNA) has never been performed for the diagnosis of solid pancreatic tumours (SPTs).Methods
We included 68 patients with a SPT referred for EUS-FNA. CTCs were analysed using size-based platform and ctDNA using digital PCR. The sensitivity, specificity, negative and positive predictive values were evaluated for each marker and their combination.Results
SPTs corresponded to 58 malignant tumours (52 pancreatic adenocarcinoma (PA) and 6 others) and 10 benign lesions. The sensitivity and specificity for PA diagnosis were 73% and 88% for EUS-FNA, 67% and 80% for CTC, 65% and 75% for ctDNA and 79% and 93% for CA19.9, respectively. The positivity of at least 2 markers was associated with a sensitivity and specificity of 78% and 91%, respectively. CtDNA was the only marker associated with overall survival (median 5.2 months for ctDNA+ vs 11.0 months for ctDNA-, P=0.01).Conclusions
CA19.9 alone and in combination with ctDNA and/or CTC analysis may represent an efficient method for diagnosing PA in patients with SPTs. Further studies including a larger cohort of patients with both malignant and benign lesions will be necessary to confirm these promising results.
SUBMITTER: Sefrioui D
PROVIDER: S-EPMC5625666 | biostudies-literature | 2017 Sep
REPOSITORIES: biostudies-literature
Sefrioui David D Blanchard France F Toure Emmanuel E Basile Paul P Beaussire Ludivine L Dolfus Claire C Perdrix Anne A Paresy Marianne M Antonietti Michel M Iwanicki-Caron Isabelle I Alhameedi Raied R Lecleire Stephane S Gangloff Alice A Schwarz Lilian L Clatot Florian F Tuech Jean-Jacques JJ Frébourg Thierry T Jardin Fabrice F Sabourin Jean-Christophe JC Sarafan-Vasseur Nasrin N Michel Pierre P Di Fiore Frédéric F
British journal of cancer 20170803 7
<h4>Background</h4>The direct comparison of CA19.9, circulating tumour cells (CTCs) and circulating tumour DNA (ctDNA) using endoscopic ultrasound-guided fine needle aspiration (EUS-FNA) has never been performed for the diagnosis of solid pancreatic tumours (SPTs).<h4>Methods</h4>We included 68 patients with a SPT referred for EUS-FNA. CTCs were analysed using size-based platform and ctDNA using digital PCR. The sensitivity, specificity, negative and positive predictive values were evaluated for ...[more]