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In vitro study on anti-inflammatory effects of epigallocatechin-3-gallate-loaded nano- and microscale particles.

ABSTRACT: This study aimed to develop an anti-inflammation system consisting of epigallo-catechin-3-gallate (EGCG) encapsulated in poly(lactide-co-glycolic acid) (PLGA) particles to promote wound healing.Nano- and microscale PLGA particles were fabricated using a water/oil/water emulsion solvent evaporation method. The optimal particle size was determined based on drug delivery efficiency and biocompatibility. The particles were loaded with EGCG. The anti-inflammatory effects of the particles were evaluated in an in vitro cell-based inflammation model.Nano- and microscale PLGA particles were produced. The microscale particles showed better biocompatibility than the nanoscale particles. In addition, the microscale particles released ~60% of the loaded drug, while the nanoscale particles released ~50%, within 48 hours. Thus, microscale particles were selected as the carriers. The optimal EGCG working concentration was determined based on the effects on cell viability and inflammation. A high EGCG dose (100 ?M) resulted in poor cell viability; therefore, a lower dose (?50 ?M) was used. Moreover, 50 ?M EGCG had a greater anti-inflammatory effect than 10 ?M concentration on lipopolysaccharide-induced inflammation. Therefore, 50 ?M EGCG was selected as the working dose. EGCG-loaded microparticles inhibited inflammation in human dermal fibroblasts. Interestingly, the inhibitory effects persisted after replacement of the drug-loaded particle suspension solution with fresh medium.The EGCG-loaded microscale particles are biocompatible and exert a sustained anti-inflammatory effect.

SUBMITTER: Wu YR

PROVIDER: S-EPMC5626413 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature

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In vitro study on anti-inflammatory effects of epigallocatechin-3-gallate-loaded nano- and microscale particles.

Wu Yan Ru YR Choi Hong Jin HJ Kang Yun Gyeong YG Kim Jeong Koo JK Shin Jung-Woog JW

International journal of nanomedicine 20170922

<h4>Purpose</h4>This study aimed to develop an anti-inflammation system consisting of epigallo-catechin-3-gallate (EGCG) encapsulated in poly(lactide-co-glycolic acid) (PLGA) particles to promote wound healing.<h4>Methods</h4>Nano- and microscale PLGA particles were fabricated using a water/oil/water emulsion solvent evaporation method. The optimal particle size was determined based on drug delivery efficiency and biocompatibility. The particles were loaded with EGCG. The anti-inflammatory effec ...[more]

PMID: 29026297

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Dataset Information

In vitro study on anti-inflammatory effects of epigallocatechin-3-gallate-loaded nano- and microscale particles.

Publications

In vitro study on anti-inflammatory effects of epigallocatechin-3-gallate-loaded nano- and microscale particles.

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