Sources of error in measurement of minimal residual disease in childhood acute lymphoblastic leukemia.

ABSTRACT:

Introduction

The level of minimal residual disease (MRD) in marrow predicts outcome and guides treatment in childhood acute lymphoblastic leukemia (ALL) but accurate prediction depends on accurate measurement.

Methods

Forty-one children with ALL were studied at the end of induction. Two samples were obtained from each iliac spine and each sample was assayed twice. Assay, sample and side-to-side variation were quantified by analysis of variance and presumptively incorrect decisions related to high-risk disease were determined using the result from each MRD assay, the mean MRD in the patient as the measure of the true value, and each of 3 different MRD cut-off levels which have been used for making decisions on treatment.

Results

Variation between assays, samples and si

SUBMITTER: Latham S 

PROVIDER: S-EPMC5626434 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature